NPA Causes a Block Lateral at an Early Stage of Lateral Root Developmenta
μM NPA | ||||
---|---|---|---|---|
Stage | 0 | 1 | 5 | 10 |
I | 12 | 22 | 6 | 0 |
II | 11 | 7 | 1 | 0 |
III | 6 | 7 | 0 | 0 |
IV | 6 | 2 | 0 | 0 |
V | 9 | 3 | 0 | 0 |
VI | 2 | 4 | 0 | 0 |
VII | 7 | 0 | 0 | 0 |
Total | 53 | 45 | 7 | 0 |
↵a Seedlings were grown on Murashige and Skoog agar containing 0, 1, 5, or 10 μM NPA for 10 days, after which time the roots were collected. Roots were sectioned, and the number and stage distribution of lateral root primordia within 2.5 to 20 mm from the primary root tip were recorded. Results presented here are the total number of detected primordia at each developmental stage from five individual roots.