Table 2.

Lignin Content and Composition in the Wild Type and the fra2 Mutant

Klason LigninaLignin Composition (mg/g Cell Wall)b
Plant% of Cell Wallc% of Wild-Type Klason LigninGuaiacyl LignincSyringyl LignincS/Gd
Wild type15.8 ± 0.410034.0 ± 0.3 (100%)15.6 ± 1.4 (100%)0.46
fra2 14.4 ± 0.69114.2 ± 4.2 (42%)10.2 ± 3.6 (65%)0.72
  • a Klason lignin was assayed according to Kirk and Obst (1988).

  • b Lignin monolignol composition was analyzed accroding to Akin et al. (1993).

  • c Each data point is the mean ±se of two separate assays. Data in parentheses are guaiacyl or syringyl lignin units taken as either 100 in the wild type or a percentage of the wild type in fra2.

  • d G (guaiacyl) is the sum of vanillin, acetovanillin, and vanillic acid; S (syringyl) is the sum of syringaldehyde, acetosyringaldehyde, and syringic acid.