Table 3.

Leaf Carotenoid Content and Xanthophyll Cycle Activitya

Postillumination DES (%)
LinesCarotenoid/Chlorophyll (mol∙mol–1)XC (% Total Carotenoid)2 Min23 Min
Wild type
$#x00A0;$#x00A0;$#x00A0;$#x00A0;HL0.30 ± 0.0117.9 ± 0.5NDbND
$#x00A0;$#x00A0;$#x00A0;$#x00A0;LL0.24 ± 0.0115.9 ± 0.612.0 ± 3.329.6 ± 4.1
CP29
$#x00A0;$#x00A0;$#x00A0;$#x00A0;HL0.27 ± 0.0115.6 ± 0.9cNDND
$#x00A0;$#x00A0;$#x00A0;$#x00A0;LL0.25 ± 0.0112.9 ± 0.3d$#x00A0;$#x00A0;9.4 ± 2.430.4 ± 4.4
CP26
$#x00A0;$#x00A0;$#x00A0;$#x00A0;HL0.28 ± 0.0117.4 ± 0.8NDND
$#x00A0;$#x00A0;$#x00A0;$#x00A0;LL0.25 ± 0.0115.1 ± 0.610.4 ± 3.133.3 ± 4.7
  • a Carotenoids and chlorophylls extracted from leaf discs from dark-adapted or light-treated plants (grown under HL or LL) were assayed by reverse-phase HPLC. The deepoxidation state (DES) of the XC pigments zeaxanthin (Z), antheraxanthin (A), and violaxanthin (V) was calculated as (Z + 1/2 A)/(Z + A + V), for leaves exposed to 1000 μmol quanta∙m–2∙sec–1 in CO2-saturating conditions for either 2 or 23 min; the preillumination DES was <4% for all lines. Data are means ±se, n = 4.

  • b ND, not determined.

  • c Significant difference of P < 0.05 from the corresponding wild-type parental line.

  • d Significant difference of P < 0.01 from the corresponding wild-type parental line.