Table 2.

HPLC Analysis of Changes in Pigment Composition Induced by High Light Exposure

DLbHLbHL + Darkb
PigmentsaWild TypeΔycf9Wild TypeΔycf9Wild TypeΔycf9
Neoxanthin7.06.16.46.06.35.9
Violaxanthin6.510.35.14.46.36.1
Antheraxanthin0.00.10.03.90.83.8
Zeaxanthin0.30.51.78.90.77.6
β-Carotene3.73.14.33.73.24.8
Lutein13.716.314.220.114.317.7
VAZ6.810.96.817.27.817.0
(Z + 0.5A)/(VAZ)0.0440.0500.330.630.140.56
Chlorophyll a71.273.875.267.076.674.9
Chlorophyll b28.826.224.833.023.425.1
Total carotenoids31.236.431.747.031.646.1
  • a Pigment compositions were normalized to 100 moles of total (a + b) chlorophyll. VAZ, V + A + Z. The ratio (Z + 0.5A)/(VAZ) refers to the de-epoxidation state as defined by Demmig-Adams and Adams (1992).

  • b Pigment compositions were measured in leaves of tobacco plants grown at 20°C under 10 μE·m−2·sec−1 (DL), exposed to high light (HL, 900 μE·m−2·sec−1) for 30 min, and allowed to recover in darkness for 10 min (HL + Dark). Analyses were performed by HPLC of 80% acetone leaf extracts.