Quantitation of GAs in Different Mutants and Wild-Type Plants
| GA | ga1-3 | rga-2/ga1-3 | spy-9/ga1-3 | Ler | rga-2/GA1 | spy-9/GA1 |
|---|---|---|---|---|---|---|
| GA53 | Undetectable | Undetectable | Undetectable | 4.2 | 3.9 | 6.5 |
| GA44 | Trace | Trace | Trace | 1.6 | 0.7 | 2.7 |
| GA19 | 1.6 | 3.2 | 2 | 6.9 | 10.4 | 8.4 |
| GA20 | 0.1 | 0.2 | 0.1 | 0.3 | 0.4 | 0.3 |
| GA1 | 0.1 | Trace | 0.1 | 0.4 | 0.4 | 0.5 |
| GA29 | 0.1 | 0.1 | 0.1 | 0.3 | 0.3 | 0.3 |
| GA8 | 2.4 | 0.4 | Trace | Trace | 1.9 | Trace |
| GA12 | Trace | 0.9 | 0.5 | 44.4 | 42.0 | 56.2 |
| GA15 | 0.7 | 0.6 | 0.6 | 7.2 | 8.3 | 10.4 |
| GA24 | Trace | Trace | Trace | 40.6 | 41.3 | 46.0 |
| GA9 | Trace | Trace | Trace | 2.3 | 2.5 | 1.7 |
| GA4 | Undetectable | Undetectable | Undetectable | 22.5 | 18.6 | 15.3 |
| GA34 | 0.4 | 0.3 | 0.4 | 6.4 | 5.5 | 4.3 |
a Values are in ng of GA/g dry weight. Trace indicates <0.1 ng of GA/g dry weight. Undetectable indicates that no corresponding peak was detected by GC-SIM. The GAs in the top half of the table are part of the early 13-hydroxylation
pathway: GA53→GA44→GA19→GA20→GA1→GA8.
↓
GA29
Those in the bottom half are part of the non-13-hydroxylation pathway: GA12→GA15→GA24→GA9→GA4→GA34. GA1 and GA4 are bioactive GAs, whereas the other GAs are either precursors or deactivated GAs (GA29, GA8, and GA34).