Table 2.

DNA Gel Blot Analysis of AIF Probes That Showed AFLP Band Disappearance in the F1 Hybrid and/or in the Allotetraploid Plants of Cross B (SlSl × AA)

ProbePrimer PairsFragment Size (bp)Origin of FragmentEliminationaCopy NumberSequence Similarityb
AIF1E+AAC/M+CAG420urartu+LowNone
AIF2E+AAC/M+CAG202urartu+LowNone
AIF3E+AAC/M+CAG150urartu+LowNone
AIF4E+AAC/M+CAG80urartuNDLowNone
AIF5E+AGG/M+CTT530longissimaNDHighNone
AIF6E+AGG/M+CTT510urartu+LowNone
AIF7E+AGG/M+CTT450urartu+LowNone
AIF8E+AAC/M+CTT170longissima+LowNone
AIF9E+AAC/M+CTT165longissima+LowNone
  • a Elimination in F1 and/or the allotetraploid was validated (+) or could not be determined (ND). AIF1 to AIF7 were eliminated in the allotetraploid only, and AIF8 and AIF9 were eliminated in both F1 and the allotetraploid.

  • b Similarity was considered significant for e values ≤ 0.005.