Table 3.

Primer Sets and Methods Used to Create Site-Directed Mutations

Gene5′; 3′ Oligonucleotide PrimersTemplateMutagenesis Methoda
AtRAC7PSY142; PSY143Flower cDNA library
AtRAC8PSY128; PSY111Flower cDNA library
AtRAC10PSY130; PSY127Flower cDNA library
RacUPSY129; PSY124EST (G.B U88402)
Atrac7mS206PSY142; PSY144AtRAC71
Atrac8mS205PSY128; PSY112AtRAC81
Atrac10mS208PSY130; PSY135AtRAC101
racUmSPSY129; PSY125RACU1
Atrac7mS203PSY142; PSY143Atrac7mSS203+2061
Atrac7mS196PSY160; PSY161AtRAC73
Atrac8mS199I) PSY145; PSY146I) AtRAC82
II) PSY128; PSY111II) First-step product
Atrac10mS202I) PSY136; PSY137I) AtRAC102
II) PSY130; PSY127II) First-step product
Atrac7mSS203+206PSY142; PSY150AtRAC71
Atrac8mSS199+205PSY128; PSY112Atrac8mS1991
Atrac10mSS202+208PSY130; PSY135Atrac10mS2021
Atrac7mCaa−PSY142; PSY150AtRAC71
Atrac8mCaa−PSY128; PSY147AtRAC81
Atrac8m10C′PSY128; PSY149AtRAC81
Atrac7mV15PSY155; PSY156AtRAC73
  • a 1, direct PCR; 2, sequential PCR steps; 3, use of the QuikChange Kit.