Table 3.

Characteristics of Chlamydomonas 30S Plastid Ribosomal Proteins

Predicted Number of Amino Acid Residues and pI ValuesMolecular Mass (kD)
30S PRPPrecursor or ProproteinaTransit Peptideb or Met RemovalcMature ProteinpIdSequenceSDS-PAGEe
S1>289NA>2894.6>32.049
S2 570Met (+) 57011.0 63.257
S3 712Met (−) 71110.7 81.776
S4 257Met (−) 25611.3 29.924 to 26
S5 67351 6224.0 66.784
S6 17154 1177.0 13.310 to 11
S7 168Met (−) 16710.4 19.014 to 16
S8 f 153Met (−) 15210.1 17.1?
S9 191Met (−) 1909.8 20.918 to 19
S10 16937 1325.8 14.714 to 16
S12 133Met (−) 13211.5 14.511 to 13
S13 16429 13510.5 15.410 to 11
S14 100Met (−) 9911.3 11.6?
S15 14133 1089.9 12.4<10
S16 12828 1009.9 11.5<10
S17>105>25 8010.1 9.0<10
S18 137Met (+) 13711.9 16.314 to 16
S19 92Met (−) 9110.8 10.3<10
S20 16638 1289.9 14.211 to 13
S21 18428 1564.7 17.7?
PSRP-1f 28659 2276.1 25.4?
PSRP-3 29836 2624.1 29.334
PSRP-4f>135>53 12710.5 13.5?
PSRP-7>37828>350?>38.066
  • a Cytosolic precursor and plastid proprotein sequences were deduced from nucleic acid sequences (see Table 2).

  • b Predicted by ChloroP (Emanuelsson et al., 1999).

  • c Predicted by penultimate amino acid residues (Giglione and Meinnel, 2001).

  • d From predicted mature protein sequence.

  • e Estimated from major peptides found in the TP30 gel section by LC-MS/MS (see Table 1).

  • f Unidentified proteins by LC-MS/MS analyses.