Table 1.

Identification of 30 Lumenal Arabidopsis Proteins from the 2-D Electrophoresis Gels Shown in Figure 1

Localization and Cleavage Site Predictione
SignalP
Spot No.Molecular
 Mass (kD)pIIdentityaAccession No.bMALDI
 Percent
 Coverage
 at 50 ppmcMS/MS
 SequencedTargeting
 PathwayTargetPPredotarEukGramGram+N Terminus
20316.79.3OEC164583542
   (At4g21280)44TATC2/44P 175-VLA-DA74-AVL-AD77-ADA-IS77-ISIKVf
20816.79.1OEC16-like7267278
   (At4g05180)37TATC3/48P 0.784-AEA-IP84-AEA-IP84-IPIKVf
11, 12, 13, 1420.55.7 to 6.0OEC237443217
   (At1g06680)40TATC2/31P 0.677-ADA-AY77-ADA-AY77-ADA-AY77-AYGEAf,g,h
20112.19.6Psa-N1709825
   (At5g64040)35TATC5/81P 0.984-ASA-NA86-ANA-GV84-ASA-NA84-NAGVIf
12336.75.6Hcf1366016183
   (At5g23120)47TATC2/60P 0.978-ARA-DE78-ARA-DE78-ARA-DE78-DEQLSf,g
205 to 20630.47.2 to 7.6Putative ascorbate
   peroxidase7267543
   (At4g09010)32TATC3/36P 182-AKA-AD82-ADLIQf,g
20713.29.3Putative new
   photosystem II
     protein2809245
   (At1g03600)33TATC2/67P 168-VSA-AE68-VSA-AE68-VSA-AE68-AEDEEg
9133.65.4Putative DegQ
   protease (DegP5)2832642
   (At4g18370)11TATC3/26P 0.510-AFS-SI71-AIA-LE71-XEQXXh
1919.45.6Putative protein
   OEC23 related2244908
   (At4g15510)23TATC1/75M 0.7104-AFA-ST104-AFA-ST54-QSA-KS104-STPVFf
7117.26.0Not annotated
   OEC23 relatedAC007171SLDQFGSPQFVAD (3)TAT (KR)C4/34P 0.871-AKS-ME26-SHH-KI69-NKA-KS
20431.48.5Putative protein
   OEC23 related7019666
   (At3g55330)31TATC1/26Neither74-SFA-AE74-SFA-AE74-SFA-AE74-AESKKg
21226.37.6Putative protein
   OEC23 related7485407
   (At2g39470)54TATC3/72Neither73-LLA-EE73-LLA-EE73-LLA-EE
10819.06.5Putative protein
   OEC23 related2829916
   (At1g77090)33TATC4/35M 163-ALA-FP63-ALA-FP70-VVKQGh
11119.56.5Putative protein
   OEC23 related7594543
   (At3g56650)GSTVVLFVVSATEK (7)TATC1/75P 0.966-ISA-AR66-ISA-AR67-REVEVg,h
21017.39.3Putative isomerase6143884
   (At3g10060)50TATC2/56Both73-ASG-IL82-AEA-VS82-AEA-VS
11017.35.7Putative FKBP
   isomerase2289010
   (At2g43560)50TATC4/48Neither59-AAG-LP57-AYA-AG57-AYA-AGAGLPPf
8025.44.7Similar to FKBP
 isomerase7543908
 (At5g13410)24TATC1/29P 0.631-VAA-RL17-ALA-GT89-SQFADf
10416.05.2Putative protein2262151
   (At4g02530)23TATC1/38P 0.958-LVA-IG58-LVA-IG73-AILEAg
7017.85.5Putative protein
 OEC23 related6642664
   (At1g76450)VEAFAETLVSGLDR (3)TATC2/32P 0.880-AFA-ET80-AFA-ET80-AFA-ET80-ETNASf
11728.94.2Plastocyanin 2130261
   (At1g76100)23Sec + otherC1/32P 0.972-AMA-ME72-AMA-ME72-AMA-ME72-MEVLLf
90, 100, 11614 to 354.4 to 4.9Plastocyanin 1
   DRT1121169201
   (At1g20340)29IEVLLGGGDGSLAFIPNDFSIAK (4)Sec + otherC1/75P 168-AMA-IE68-AMA-IE68-IEVLLf,h
31 to 3328.55.1 to 5.6OEC333286693
   (At5g66570)55Sec + otherC2/26P 0.885-ASA-EG85-ASA-EG85-ASA-EG85-EGAPKf
OEC33-like4835233
 (At3g50820)37Sec + otherC2/28P 0.984-AGA-EG84-AGA-EG84-AGA-EG84-EGAPKf
12736.56.2DegP12565436
   (At3g27925)31Sec + otherC3/35P 1100-VES-AS99-AVE-SE100-VES-AS103-FVVSTf
9538.54.8TLP40 rotamase6016707
   (At3g01480)53Sec + otherC1/75P 182-AHA-VA82-AHA-VA82-AHA-VA92-VLISGf
21121.28.5FKBP-like protein6686798
   (At4g39710)32Sec + otherC3/74M 0.673-ADA-TR73-ADA-TR73-ADA-TR
10716.75.3Putative protein
   pentapeptide repeat6226234
   (At5g53490)36AFVGNTIGQADGVYDKPLDLR (1)Sec + otherC4/65P 0.777-VIA-AN77-VIA-AN77-VIA-AN77-ANQRLg
10315.05.7Putative protein
   pentapeptide repeat2344892
   (At2g44920)31Sec + otherC1/56P 0.881-ALA-FK81-ALA-FK81-ALA-FK81-FKGGGg
10616.04.8Putative protein8809586
   (At5g52970)VLAQNYPVTPGLAIK (4)Sec + otherC4/83P175-ADA-KV75-ADA-KV75-ADA-KV75-KVGVNg
7318.25.0Putative protein3776572
   (At1g54780)44Sec + otherC2/53P 184-ALA-SE84-ALA-SE84-ALA-SE84-SEFNIh
6817.15.3Putative protein4455236
 (At4g24930)FWLEDTPYGR (2)Sec + otherC4/66M 0.563-ALA-IP63-ALA-IP63-ALA-IP63-IPSLSf
  • a Identity given in NCBI completed by domain prediction found by Pfam, Blocks, Prints, Prodom, or Phi-Blast.

  • b Accession numbers in NCBI and in MIPS (in parentheses). All accession numbers in italics showed misassignments in the database and have been corrected.

  • c Percentage of coverage at 50 ppm for the MALDI-TOF peptides.

  • d Sequence tag identified by ESI/MS/MS; additional sequence tags were obtained (numbers listed in parentheses) but are not shown.

  • e Localization, reliability class, and cleavage site prediction by TargetP (C for chloroplast), localization and score by Predotar (P for plastid, M for mitochondria), cleavage site prediction of the lumenal transit peptide by three versions of SignalP developed for eukaryotes (Euk), Gram-negative or Gram-positive bacteria, and in boldface when this prediction fits with the N-terminal Edman sequence.

  • f N-terminal Edman sequence tag found in the literature or in public databases.

  • g N-terminal Edman sequence tag identified in our previous study of pea.

  • h N-terminal Edman sequence tag identified in this study.

  • Proteins were identified by MALDI-TOF MS and/or nanoESI/MS/MS. N termini of all proteins were determined by Edman sequencing. All accession numbers in italics showed mis-assignments in the database and have been corrected.