Table 4.

CRTISO Enzyme Assay

CarotenoidEtiolated ccr2
 + E. coliEtiolated ccr2
 + CRTISOProportional
 Changea
All-trans-lycopene5.0 ± 0.79.5 ± 1.81.89 ↑
Prolycopene31.9 ± 3.126.3 ± 4.70.82 ↓
Neurosporene3.1 ± 0.014.8 ± 0.51.53 ↑
Proneurosporene9.9 ± 1.97.9 ± 2.50.79 ↓
All-trans-ζ-carotene1.6 ± 0.472.2 ± 0.161.41 ↑
Mono-cis-ζ-carotene7.8 ± 0.48.2 ± 0.71.05 —
Pro-ζ-carotene10.6 ± 0.88.9 ± 1.30.84 ↓
  • a Incubation of recombinant CRTISO protein with ccr2 etiolated tissue resulted in a decrease in prolycopene, proneurosporene, and pro-ζ-carotene with a similar increase in the respective all-trans isoforms. Averages and standard deviations for four experiments are provided.