Table 3.

Functional Expression of CRTISO in E. coli

GenesPhytoenePhytoflueneζ-CaroteneNeurosporeneDi-cis-lycopeneProlycopeneLycopeneOthers
Light
  crtE, crtB98.41.6
  crtE, crtB, Zds, CRTISO99.40.6
  crtE, crtB, crtP12.2 ± 1.86.8 ± 0.279.2 ± 1.51.8
  crtE, crtB, crtP, CRTISO15.2 ± 4.67.0 ± 0.675.3 ± 4.72.5
  crtE, crtB, crtP, Zds17.9 ± 0.110.4 ± 0.254.6 ± 0.63.6 ± 0.26.9 ± 0.76.2 ± 0.50.4
  crtE, crtB, crtP, Zds, CRTISO19.7 ± 3.111.4 ± 1.238.5 ± 5.630.1 ± 9.70.3
Dark
  crtE, crtB99.01.0
  crtE, crtB, Zds, CRTISO100
  crtE, crtB, crtP8.9 ± 1.56.0 ± 0.883.2 ± 2.21.9
  crtE, crtB, crtP, CRTISO11.7 ± 0.97.6 ± 0.178.5 ± 1.32.2
  crtE, crtB, crtP, Zds13.7 ± 1.211.1 ± 1.666.2 ± 1.98.3 ± 1.40.7
  crtE, crtB, crtP, Zds, CRTISO23.2 ± 1.116.5 ± 0.950.0 ± 1.90.6 ± 0.98.5 ± 2.41.2
  • Cells of E. coli, all carrying plasmid with the gene Ipi, were transfected with different combinations of carotenoid biosynthesis genes. crtE, geranylgeranyl diphosphate synthase; crtB, phytoene synthase; crtP, phytoene desaturase; Zds, ζ-carotene desaturase; CrtiSO, carotenoid isomerase. Numbers correspond to percentage of total carotenoids.