Table 1.

Changes in Primary Root Lengths of pls and Wild-Type C24 Seedlings after 7 Days of Continuous Growth on Cytokinin (BA) and ACC and after Transfer at 3 Days after Germination from Hormone-Free to Auxin (2,4-D, 1-NAA)–Containing Medium for another 3 Days

C24 (n = 10)pls (n = 10)
Mean Root Length (mm ± se)Percent Change from ControlMean Root Length (mm ± se)Percent Change from Control
1/2MS1018.0 ± 0.710011.6 ± 0.4100
BA (100 pM)17.9 ± 0.799.49.2 ± 0.479.3
BA (1 nM)17.3 ± 0.896.19.4 ± 0.481.0
BA (10 nM)16.9 ± 0.893.98.3 ± 0.471.6
BA (100 nM)9.6 ± 0.253.34.9 ± 0.342.2
BA (1μM)9.0 ± 0.350.03.3 ± 0.328.4
ACC (10 pM)14.6 ± 0.881.18.9 ± 0.376.7
ACC (100 pM)11.3 ± 0.562.87.4 ± 0.463.8
ACC (1 nM)7.2 ± 0.240.04.8 ± 0.341.4
ACC (10 nM)3.5 ± 0.219.41.9 ± 0.216.4
1/2MS1036.0 ± 1.010011.9 ± 0.7100
2,4-D (100 pM)32.8 ± 0.691.115.1 ± 0.7126.5
2,4-D (1 nM)30.5 ± 0.884.612.9 ± 0.6108.4
2,4-D (10 nM)7.6 ± 0.4215.5 ± 0.346
2,4-D (100 nM)2.3 ± 0.16.41.8 ± 0.115.1
NAA (100 pM)33.6 ± 0.893.315.1 ± 0.5126.9
NAA (1 nM)34.5 ± 0.695.814.4 ± 0.6120.6
NAA (10 nM)30.3 ± 0.68412.7 ± 0.3106.3
NAA (100 nM)11.4 ± 0.331.75.1 ± 0.242.9
NAA (250 nM)5.6 ± 0.115.63.4 ± 0.128.2
  • The relative change in primary root length is shown as percent change compared with control seedlings grown on hormone-free medium (1/2MS10). The seedings treated with BA and ACC were grown on horizontal agar plates, whereas the seedlings treated with auxins were grown on vertical agar plates, accounting for root length differences between controls of the respective treatments.