Table 1.

Specificity of PHT2;1

Reagent33Pi Uptake Activity (%)
Water control100
K2SO4 (10 mM)99
KNO3 (10 mM)97
KCl (10 mM)96
KH2PO4 (0.5 mM)62
KH2PO4 (2.5 mM)34
  • Anions tested as potential competitors in Pi uptake assays were added as potassium salts to yeast PAM2 cells expressing PHT2;1(Δ1-71), 30 s before the addition of labeled Pi (0.25 mM final concentration). Values were derived from three independent measurements per treatment with se < 3%.