Table 3.

Identification of Products Formed by the Incubation of Recombinant GA 2-Oxidase (AtGA2ox7) with GA12 or GA53

SubstrateProductMass Spectra of Productsa [mass-to-charge ratio (% relative abundance)]
−14C4-GA12−14C4-GA110M+ 456 (11), 448 (8), 441 (11), 433 (8), 424 (51), 416 (44), 396 (78), 388 (66), 379 (7), 373 (7), 322 (13),
 316 (10), 306 (100), 298 (92), 291 (71), 283 (64), 262 (27), 261 (28), 258 (23), 257 (26), 245 (98), 239 (74),
 229 (37), 223 (24), 199 (16), 197 (12), 147 (37), 145 (41)
−14C4-GA53−14C4-GA97M+ 544 (45), 536 (22), 529 (12), 521 (5), 510 (5), 504 (4), 483 (13), 477 (6), 452 (3), 446 (2), 393 (8), 387 (4),
 377 (5), 371 (4), 331 (5), 327 (5), 301 (5), 297 (3), 243 (19), 239 (17), 210 (73), 209 (100), 208 (31), 207 (56)
  • a As the methyl ester trimethylsilyl ethers.