Table 2.

Properties of Petunia BSMTs

EnzymeHoloenzyme
 Mass by Gel
 Filtration (D)Subunit
 Mass by
 SDS-PAGE (D)Calculated
 Subunit
 Mass (D)SubstratepH
 OptimumApparent Km
 for Substrate
 (μM)kcat × 10−2 (s−1)kcat/Km (mM−1·s−1)
BSMT178,430∼45,00040,659SAM SA1.93 ± 0.181.45 ± 0.0247.65 ± 0.618
SAM BA15.78 ± 1.340.75 ± 0.0230.48 ± 0.047
Salicylic acid7.0 to 8.051.55 ± 2.731.2 ± 0.160.23 ± 0.038
Benzoic acid7.51273.2 ± 370.39 ± 0.040.003 ± 0.0003
BSMT277,100∼45,00040,673SAM SA2.76 ± 0.040.57 ± 0.0992.07 ± 0.015
SAM BA30.78 + 0.780.79 ± 0.060.26 ± 0.015
Salicylic acid7.0 to 7.569.6 ± 1.310.4 ± 0.0280.057 ± 0.005
Benzoic acid6.5 to 7.52355.9 ± 880.32 ± 0.00960.0014 ± 0.0084