Effects of Microbeam Irradiation on Cytoplasmic Motility in Epidermal Cells of Vallisneria
| Effect | Experiment 1 | Experiment 2 | Experiment 3 |
|---|---|---|---|
| Irradiated cell or region | 20.5 ± 11.3a | 30.2 ± 23.8a | 25.0 ± 10.1a |
| Adjacent cells or regions | |||
| Distal | 13.5 ± 4.9 | 13.6 ± 4.0 | 16.1 ± 5.6 |
| Proximal | 14.5 ± 5.4 | 14.2 ± 3.5 | 12.6 ± 4.5 |
| Right side | 13.8 ± 6.3 | 13.3 ± 4.0 | 11.3 ± 4.7 |
| Left side | 12.9 ± 6.5 | 14.0 ± 3.6 | 12.0 ± 4.4 |
| Distant cell or region | 14.2 ± 5.1 | 13.5 ± 3.4 | 14.8 ± 4.2 |
| Diameter of microbeam | 50 μm | 10 μm | 5 μm |
| Duration of irradiation | 5 s | 1 s | 1 s |
| Number of pixels examined | 3363 | 225 | 49 |
↵a Significantly different from other cells or regions (P < 0.001).
A part of one epidermal cell in a dark-adapted sample was irradiated with red light of 100 μmol·m−2·s−1 using the microirradiation system. The index of cytoplasmic motility was determined immediately after the termination of actinic irradiation on the irradiated cell or region and several neighboring cells or regions in the same optical field. For each experiment, the responses are expressed as the average of the index of cytoplasmic motility obtained from pixels in a defined area with the standard deviation. In experiment 3, the magnitude of response was compared between the irradiated region and the adjoining unirradiated regions in the same cell.