Table 3.

Effects of Microbeam Irradiation on Cytoplasmic Motility in Epidermal Cells of Vallisneria

EffectExperiment 1Experiment 2Experiment 3
Irradiated cell or region20.5 ± 11.3a30.2 ± 23.8a25.0 ± 10.1a
Adjacent cells or regions
 Distal13.5 ± 4.913.6 ± 4.016.1 ± 5.6
 Proximal14.5 ± 5.414.2 ± 3.512.6 ± 4.5
 Right side13.8 ± 6.313.3 ± 4.011.3 ± 4.7
 Left side12.9 ± 6.514.0 ± 3.612.0 ± 4.4
 Distant cell or region14.2 ± 5.113.5 ± 3.414.8 ± 4.2
Diameter of microbeam50 μm10 μm5 μm
Duration of irradiation5 s1 s1 s
Number of pixels
   examined336322549
  • a Significantly different from other cells or regions (P < 0.001).

  • A part of one epidermal cell in a dark-adapted sample was irradiated with red light of 100 μmol·m−2·s−1 using the microirradiation system. The index of cytoplasmic motility was determined immediately after the termination of actinic irradiation on the irradiated cell or region and several neighboring cells or regions in the same optical field. For each experiment, the responses are expressed as the average of the index of cytoplasmic motility obtained from pixels in a defined area with the standard deviation. In experiment 3, the magnitude of response was compared between the irradiated region and the adjoining unirradiated regions in the same cell.