Table 2.

Expression Profiles of Tunicamycin-Upregulated Genes and the Corresponding Results in the DTT Experiment

Tunicamycin FVbDTT
02+25+502+25+5
BACAffymetrix CodeDescriptionTagaFVFcFVFFVFFVFFVF
Protein folding
chaperones
T12M4.813067_s_atCalreticulin2OL-LS1.04.30.98.81.01.0P2.1P1.0P2.3P0.7P
F7J7.12015026_atSimilar to heat shock dnaJ homologOL-HS0.91.80.83.71.01.0P2.2P0.8P2.8P0.8P
D89341/215172_s_atLuminal binding protein, BiPOL-HS0.93.70.84.51.01.0P4.3P1.0P4.6P0.8P
T19F6.116448_g_at (D2T2)dHSP90 isologOL-HS0.83.60.77.61.01.0P2.3P0.9P2.9P0.9P
MAC9.1117473_atCalnexin, CNX1OL-HS1.02.61.05.90.91.0P5.2P0.9P4.3P1.0P
T2I1.5017924_atCalnexin homologOL-HS1.03.70.96.71.01.0P4.0P0.9P3.1P1.0P
Disulfide bond formation
F28P10.6014036_atPDI-like proteinNOL1.01.10.93.10.81.1P1.0P1.0P1.6P0.8P
F8K7.1915976_atSimilar to PDINOL0.82.10.94.51.01.0P1.4P0.9P1.6P0.8P
T30B22.2317398_atPutative PDIOL-HS1.01.70.65.00.81.0P1.8P0.9P3.3P1.0P
T21L14.1419457_atPutative PDIOL-HS0.93.21.06.80.61.0P3.9P0.8P5.6P1.0P
Translocon/protein degradation
T18E12.2112042_atSignal peptide peptidase-likeOL-LS1.01.90.95.40.91.0P1.6P1.0P2.2P0.9P
F13P17.913102_atPutative protein transport protein SEC61 α-subunitNOL0.92.60.83.31.00.9P1.3P1.0P1.7P0.9P
T14P1.1216950_s_at (D2T2)dsec61 β-subunit homologOL-HS1.04.30.92.70.71.0P2.4P0.8P2.6P1.0P
F13M23.6017882_atProtein transport protein sec61 γ-subunit-likeOL-HS1.03.50.93.60.91.0P3.6P1.0P4.2P1.0P
F8D23.320475_atPutative AAA-type ATPaseOL-LS1.01.00.93.31.20.6A1.8P0.6P4.3P1.0P
Glycosylation/modification
T3D7.113134_s_atPutative galactinol synthaseOL-LS1.00.70.92.51.01.0P1.6P1.0P4.4P0.8A
F4I18.2314061_atPutative phosphomannomutaseNOL1.01.01.02.80.60.9P1.6P1.0P1.7P1.0P
T20F6.514381_atUDP-galactose transporter, AtUtr1OL-HS1.05.00.68.70.70.9P6.6P1.0P6.8P1.0P
T12J13.817109_s_atβ-GlucosidaseNOL1.00.91.111.90.91.2P0.9P1.0P1.0P1.0P
T32G6.118675_at (D1T2)dGPTOL-HS1.03.20.46.10.90.8P1.5P1.0P3.1P0.9P
Vacuolar
F4F15.30013123_atSimxilar to St12p proteinOL-HS0.81.01.02.60.61.0P2.0P1.0P2.6P0.9P
F9K20.220229_atSimilar to vacuolar H+-pyrophosphataseOL-LS1.01.50.92.70.90.8P1.1P1.0P2.1P0.8P
Vesicle/trafficking
F12F1.2719212_atSimilar to vesicle trafficking proteinOL-HS0.91.91.03.70.71.0P1.8P0.8P3.2P0.8P
F8K4.2119648_atSimilar to coatomer α-subunit (HEPCOP) homologOL-HS1.01.91.02.80.91.0P2.9P1.0P3.5P1.0P
F7A19.1020069_atPutative Golgi-associated membrane trafficking proteinNOL0.90.81.03.31.00.9P0.9P1.0P1.4P1.0P
F13D4.7014078_atSimilar to mouse stromal cell-derived factor 2OL-HS1.02.61.03.91.00.9P2.6P0.8P3.3P1.0P
Kinases
F9L1.4215438_atSer/Thr kinase receptor-associated proteinNOL1.02.60.81.41.02.1P0.9P1.4P0.4P1.0P
F22O13.1319692_atPutative calcium-dependent protein kinaseOL-HS1.00.62.03.30.90.4P1.0P0.6P2.5P1.4P
Jasmonate related
F9D16.7014832_atTyrosine transaminase-like proteinNOL1.02.80.90.91.30.5P1.2P1.0P0.7P1.0P
T02O04.715996_atJasmonate-inducible protein isologNOL1.03.71.31.00.62.5P0.2A1.4P0.0A1.0P
Stress related
T17M13.1617014_s_atSecreted ribonuclease 1, RNS1NOL0.82.51.01.81.01.2P0.6P1.0P0.5P1.0P
F22H5.1817865_atIsoflavonoid reductase homologNOL1.00.91.12.50.90.8P1.6P1.3P1.0P0.9P
F1N13.11018699_i_at (T3)dCold-regulated, cor6.6NOL1.01.80.33.11.00.8P1.7P1.0P1.6P1.0P
T20B5.818954_atPutative cysteine proteinase inhibitor BNOL1.01.41.06.20.71.0P1.0P0.9P1.4P1.1P
F14N23.2520060_atSimilar to glutathione S-transferase TSI-1OL-LS1.02.51.01.80.71.0P1.8P0.8P2.3P1.0P
Transcription factors
MXC7.613297_atAuxin-responsive protein IAA2NOL1.20.81.03.00.71.5P1.5P0.7A1.0P0.5P
F17L21.1420032_atProtein with squamosa promoter binding domainOL-HS1.03.01.05.50.71.0P3.8P1.0P4.0P1.0P
Chloroplast residents
F27I1.217054_s_atLhcb4:3 proteinNOL0.82.01.02.70.70.6P1.3P1.0P1.2P0.8P
F11A3.1918665_r_atPhotosystem I subunit IV precursorNOL1.51.00.92.90.81.7P1.0A0.9A1.0A1.4P
FCAALL.518670_g_atChloroplast-targeted β-amylase enzymeNOL1.11.01.02.50.81.0P1.0P0.9P0.2P1.2P
Unclassified
T20K18.7013177_atGrowth factor-like protein with mutT domainOL-HS0.81.41.03.00.91.0P8.1P0.7P14.4P0.7P
T8O5.2013591_at (D2T2)dSimilar to NADH dehydrogenase chain 4OL-HS1.03.60.76.20.61.0P4.1P0.7P5.8P0.9P
T20G20.215004_atPutative microtubule-associated proteinNOL1.01.20.82.90.90.7P1.2P1.0P1.8P0.8P
F13H10.2215839_atUnknown proteinOL-HS0.71.40.94.31.01.0P3.4P0.5P10.4P0.5P
T16L4.3016311_atUnknown proteinOL-LS0.85.91.016.10.90.8A6.5P1.0P6.1P0.9P
T4L20.21020178_atUnknown proteinOL-HS1.04.61.05.40.90.8P8.8P0.9P4.4P1.0P
  • a OL-HS, overlapping genes at high-stringency criteria; OL-LS, overlapping genes at low-stringency criteria; NOL, no overlapping genes.

  • b FV, fold variation. Boldface numbers indicate genes meeting the 1000-5P-2.5 criteria. Italicized boldface numbers indicate genes that were identified when lower stringency criteria were used (1000-3P-2).

  • c F, absolute call flags: P, present; M, marginal; A, absent.

  • d Numbers of replicas on the Affymetrix GeneChip for this gene meeting the restrictions in tunicamycin (T) and DTT (D) are shown in parentheses.