Table 2.

List of the PCR-Based Molecular Markers Developed in This Study

MarkerPrimer PairsaFragment SizebRestriction Enzyme
36N13fF, 5′-AATCTTCTCTTACTCCACTCGC-3′; R, 5′-ATGGGAACTACTGACTAAACCG-3′C = 489, b = 0
57M10rF, 5′-AGGAACAGATGGAATCTCAGG-3′; R, 5′-TGTCTCCTGTCCCAAACTAGC-3′C = 340, b = 0
52G19fF, 5′-GCACGCATTTAAGAAGCAGG-3′; R, 5′-TTATATGACCGTATGGCAGG-3′502MnlI
P1F, 5′-TTGCGTATGTCTGTCAACTGC-3′; R, 5′-TTCCAACATCTGAACCCTCG-3′C = 1580, b = 0
P2F, 5′-GTTGCTCATCGTCACCATCG-3′; R, 5′-AGGTATAGAGCGAGCGGTAGC-3′1037NsiI
P3F, 5′-GGTAGTTGAAGTTTGTGATGGC-3′; R, 5′-ATGCTCTCTCCTCGCTCTGC-3′1110BstNI
P4F, 5′-TACTACAACGACCTGCTTATGG-3′ R, 5′-TGGAGAGCAGTGTAGGTAGGG-3′828FokI
P5F, 5′-AGCAGCAGTAGTCGCAGTCG-3′; R, 5′-AACATAGCCATCTGGGGTCC-3′1762MnlI
P6F, 5′-TAGAAACCAGGGTCAAGTAGGC-3′; R, 5′-GCTGAGTTTGAGGTCGGTGG-3′1470MseI
P7F, 5′-AGTGCCTCATCTATCGCTTCG-3′; R, 5′-GTTCCACAAACCGATCTAGCG-3′2046ScaI
  • a F, forward primer; R, reverse primer.

  • b Numbers indicate the size (in bp) of amplified fragments. b, bc1-2 mutant; C, C-Bao.