Table 1.

Identities and Expression Patterns of the Sequence Tags Isolated for Their Differential Expression in the Wild Type and pal Mutants by cDNA-AFLP Transcript Profiling

Expression inNearest Homolog (PEDANT at MIPS)
AGIWTpal1pal2DMDescription (MIPS)AGI/GIDescriptionE Value
Amino Acid–Related
At3g049400010Cys synthase (AtcysD1)
At5g179200010Met synthase
At1g695700011H-protein promoter binding factor-2b, putativeAt5g39660Promoter-binding protein like1 × 10−118
At3g154500011Unknown proteinAt5g10240Asn synthetase ASN33 × 10−71
At4g330100100P-Protein-like proteinGI:20471026Gly dehydrogenase, Homo sapiens0
At2g260800111Putative Gly dehydrogenaseGI:20471026Gly dehydrogenase, H. sapiens0
At2g278200111Putative chorismate mutase/prephenate dehydratase
At5g548100111Trp synthase β chain 1 precursor
Carbohydrate-Related
At2g211700010Putative triosephosphate isomerase
At1g329000100Granule-bound starch synthase-like protein
At5g174200101Cellulose synthase catalytic subunit (IRX3)
At1g355800111Invertase, putative
At3g485300111Unknown proteinGI:7300717SNF4Aγ gene product, Drosophila melanogaster5 × 10−64
At5g580900111β-1,3-glucanase 6
At3g469701011Starch phosphorylase H (cytosolic form)-like protein
Phenylpropanoid-Related
At3g532600100Phe ammonia-lyase (AtPAL2)
At3g022800100Putative NADPH-ferrihemoprotein reductase ATR3At4g24520NADPH-ferrihemoprotein reductase ATR10
At1g5168001014-Coumarate:CoA ligase (At4CL1)At4g051604-Coumarate:CoA ligase-like protein1 × 10−147
At1g436200111Hypothetical proteinGI:6562348UDP-glucoronosyl/glucosyl transferase, Caenorhabditis elegans1 × 10−124
At2g325200111Putative carboxymethylenebutenolidase
At1g491401000Similar to NADH-ubiquinone oxidoreductase precursor
Light-Related
At2g432800010Unknown proteinAt5g28530Far-red-impaired response protein (FAR1)-like3 × 10−52
At1g650700111(No name given)GI:33876770mutS protein homolog 5, H. sapiens1 × 10−150
At1g530901101Phytochrome A suppressor SPA1, putativeAt4g11110COP1-like protein2 × 10−71
At3g221701101Far-red-impaired response protein, putativeAt5g28530Far-red-impaired response protein (FAR1)-like1 × 10−141
Stress Response
At3g111700111ω-3 Fatty acid desaturase, chloroplast precursor
At4g160700111Unknown proteinAt3g49050Calmodulin binding heat shock–like protein9 × 10−61
At4g168600111Disease resistance RPP5-like protein
At5g426500111Allene oxide synthase
At5g621300111Unknown proteinGI:83226PER1, Saccharomyces cerevisiae1 × 10−99
Signal Transduction/Transcriptional Regulation
At3g115000010Putative small nuclear ribonucleoprotein polypeptide G
At1g074100011Small G protein, putative
At5g572100011Microtubule-associated protein-likeGI:12188746Rab6 GTPase-activating protein, H. sapiens4 × 10−50
At1g043900100Hypothetical proteinGI:6705975KIAA0740 protein, H. sapiens2 × 10−54
At1g219200101Phosphatidylinositol-4-phosphate 5-kinase-like protein
At1g232300110Conserved hypothetical proteinGI:7291500CG3695 gene product, D. melanogaster0
At1g233800111Knotted-like homeobox protein, putativeAt5g25220KNAT3 homeodomain protein1 × 10−44
At3g284500111Receptor kinase, putativeAt5g16000Receptor protein kinase-like protein1 × 10−117
At3g615700111Putative proteinGI:29387238CDC42 binding protein kinase β, H. sapiens2 × 10−29
At4g180100111Putative inositol polyphosphate 5-phosphatase At5P2GI:21396493Inositol polyphosphate 5-phosphatase, H. sapiens1 × 10−101
At5g146700111ADP-ribosylation factor-like protein
At2g410901000Calcium binding protein (CaBP-22)At4g04710Putative calcium-dependent protein kinase5 × 10−34
At1g730801011Unknown proteinAt5g07280Receptor-like protein kinase-like protein0
At2g216601011Gly-rich RNA binding protein
At3g123001110Unknown proteinGI:7297748CG17118 gene product, D. melanogaster5 × 10−48
Transport
At5g035550100Uracil transporter-like protein
At5g022700101ABC transporter-like protein
At4g349500111Putative proteinGI:16411641Strongly similar to transmembrane transporter2 × 10−34
At3g080401000Putative integral membrane protein
Unknown
At1g280700011Hypothetical protein
At1g287000011Unknown protein
At1g758100011Hypothetical protein
At2g400800011Unknown protein
At3g260000011Unknown protein
At4g323400011Unknown protein
At4g120700101Unknown protein
At1g672100110Unknown protein
At1g733300111Putative protein
At3g478330111Unknown protein
At3g512500111Unknown protein
At4g270801000Unknown protein
At4g142601011Hypothetical protein
At3g018601101Unknown protein
  • The description of the genes is retrieved from the Munich Information Center for Protein Sequences (MIPS); when the gene was annotated as unknown, the closest homolog is given as obtained from PEDANT at MIPS (Frishman et al., 2003). Expression was scored in the wild type and pal1, pal2 and pal1 pal2 double mutants (DM) with 0 for absence/decrease and 1 for presence/increase in expression, taking the wild type as reference. AGI, Arabidopsis Genome Initiative.