Table 3.

Soluble Phenolics That Accumulate Differentially in the Inflorescence Stems of the Wild Type and pal Mutants as Resolved by HPLC

Peak Height in μV/μg Dry Weight (Average with se)
RT (min)aIdentityWild Typepal1pal2pal1 pal2
Total99.87 ± 6.6573.98 ± 8.43106.01 ± 12.9534.14 ± 5.73*
6.033Glycosylated vanillic acid5.89 ± 0.295.65 ± 0.196.08 ± 0.992.26 ± 0.34*
7.331Scopolin0.67 ± 0.070.41 ± 0.02*0.64 ± 0.090.19 ± 0.04*
8.227Glc-Glc-Rha-kaempferol19.12 ± 2.0712.95 ± 2.2117.49 ± 2.620.21 ± 0.08*
8.350Not identifiedNDNDND0.03 ± 0.01
10.005Glc-Rha-kaempferol20.67 ± 2.5912.61 ± 3.3519.39 ± 3.310.31 ± 0.10*
11.178FM(4–O–8)Gb1.21 ± 0.150.62 ± 0.06*0.83 ± 0.110.22 ± 0.05*
11.386Rha-Rha-kaempferol25.89 ± 2.3219.71 ± 3.0227.66 ± 3.700.64 ± 0.23*
15.119FM(5–8)Gc1.34 ± 0.130.74 ± 0.09*1.16 ± 1.270.42 ± 0.09*
  • The 106 μV corresponds to an absorbance of 1 OD. One-way ANOVA and post-hoc least square difference tests for peak height were performed (see Methods) and are marked by an asterisk, if found significantly different from the wild type at P = 0.05. ND, not detected.

  • a The RTs correspond to those in an individual pal1 chromatogram, shown in Figure 8.

  • b Feruloyl malate coupled 4–O–8 to coniferyl alcohol.

  • c Feruloyl malate coupled 5–8 to coniferyl alcohol.