Table 4.

Concentrations of Amino Acids in 3-Month-Old Inflorescence Stems of the Wild Type and pal1, pal2, and pal1 pal2 Mutants as Determined by HPLC

Peak Height in μAU/μg Dry Weight (Average with se)
Amino AcidRT (min)Wild Typepal1pal2pal1 pal2
Asp3.75567.0 ± 65.6741.3 ± 35.2722.7 ± 38.7930.7 ± 182.6
Glu4.001185.8 ± 133.81744.0 ± 42.11745.2 ±83.32728.5 ± 527.3*
Asn6.05NDNDND4.2 ± 4.2
Ser6.20117.0 ± 24.7147.7 ± 5.7184.3 ± 11.4177.6 ± 25.8
Gln6.4878.3 ± 16.1135.8 ± 4.6211.2 ± 29.8741.2 ± 149.0*
Gly6.651.1 ± 1.1NDNDND
His7.208.3 ± 2.015.1 ± 3.415.9 ± 1.125.5 ± 6.1*
Arg8.10NDNDNDND
Thr8.30101.7 ± 15.3200.2 ± 5.5181.8 ± 12.4536.7 ± 98.3*
Ala8.6597.0 ± 21.2128.1 ± 2.6191.9 ± 14.0300.8 ± 40.1*
Pro8.9523.3 ± 4.758.0 ± 3.347.0 ± 6.1198.2 ± 58.8*
Tyr11.2045.0 ± 13.558.4 ± 4.650.1 ± 6.1166.0 ± 31.2*
Val11.85112.1 ± 18.3191.3 ± 5.0237.1 ± 7.6494.6 ± 78.3*
Met12.20439.1 ± 41.8522.7 ± 34.9717.1 ± 117.2*26.3 ± 3.2*
Cys12.707.2 ± 1.21.1 ± 1.1*6.8 ± 0.78.2 ± 0.8
Ile13.4540.1 ± 7.277.1 ± 0.986.4 ± 6.7161.9 ± 23.7*
Leu13.6543.6 ± 5.991.8 ± 4.7*70.3 ± 3.8103.9 ± 14.9*
Phe14.3519.9 ± 4.172.8 ± 2.460.9 ± 4.92653.3 ± 740.7*
Trp14.6227.3 ± 3.149.2 ± 3.367.4 ± 5.6121.3 ± 31.1*
Lys15.3011.8 ± 3.624.7 ± 0.8*18.2 ± 1.621.9 ± 2.6*
  • Data are average of five individuals per genotype. One-way ANOVA and post-hoc Dunnett's tests were conducted (see Methods). Values significantly different from the wild type at P < 0.05 are indicated by asterisks. ND, not detected; AU, absorption units.