Table 1.

Assay Principles, Stability to a Freeze/Thaw Cycle, Optimal Extract Dilution, Average Activity, and Average Coefficient of Variation between Biological Replicates of Rosettes from 5-Week-Old Arabidopsis Col-0 Collected at Various Time Points throughout a 12-h-Day and 12-h-Night Cycle

EnzymeMethodFreeze/Thawing Stability (% Retained ± sd)Dilution of FWAverage Activity (Arabidopsis Rosette, nmol g−1 FW min−1)Average Coefficient of Variation (%)
AGPaseG3POX/G3PDH85 ± 51,0001,25512
NAD-GAPDH90 ± 120,00036,98115
NADP-GAPDH86 ± 310,00017,04114
PFP26 ± 91,00019116
PK70 ± 91,00095218
SPSND1,0005408
TK99 ± 31,0007,46217
GK51 ± 95001833
cFBPaseG6PDH/(PMS + MTT)55 ± 2650010523
GlcK65 ± 131,00015023
FruK65 ± 111,00017918
G6PDH37 ± 95,00084913
ICDH97 ± 292,0001,50624
ShikDH48 ± 52,00059818
AlaATADH/(PES + MTT)79 ± 2120,0005,46220
AspAT81 ± 3020,0005,22919
Fumarase9 ± 320,0004,87510
GLDHam97 ± 71,00087611
PEPCase126 ± 351,00074419
Acid InvGlucose oxidase + dihydroxyphenoxazine62 ± 141,00053220
Fd-GOGATGLDH37 ± 75001,68513
NIASA + NNEDA175 ± 49 (Vsel)50012
93 ± 18 (Vmax)5758
GSPK + LDH129 ± 161,0001,86714
  • See Figure 1 for abbreviations. Acid Inv, acid invertase; cFBPase, cytosolic FBPase; GLDHam, GLDH aminating; NAD-GAPDH, NAD-dependent GAPDH; NADP-GAPDH, NADP-dependent GAPDH; MTT, methylthiazolyldiphenyl-tetrazolium bromide; NNEDA, N(1-naphtyl)ethylendiamide dihydrochloride; SA, sulfanilamide; ND, not determined; –, not determinable.