Table 1.

Summary of Microarray Analysis

Locus IdentifierWild Type +Fe Baseline SignalWild Type −Fe Chip SignalFold Changefit1-1 +Fe Baseline Signalfit1-1 −Fe Chip SignalFold ChangeAnnotation
Iron Regulation Depends Mainly on FIT1
Transporter
    At5g3882019413006.81892181.2Amino acid transporter family protein
    At4g301201447945.51621731.1HMA3, cadmium-transporting ATPase
    At4g216801225704.7120111−1.1Oligopeptide transporter (POT) family
    At1g80830153164654.2159223391.5NRAMP1, metal ion transporter
    At3g53480174052823.115121464−1.0ABC transporter-like protein
    At3g603302617803.0313125−2.5AHA7, plasma membrane H+-ATPase
    At4g330201202992.69768−1.5ZIP9, Fe(II) and Zn transport protein
Transcription Factor
    At4g09110503086.246481.0Putative RING-H2 zinc finger protein
    At3g13610213184764.023552066−1.2Similarity to DNA binding protein zyxin
    At2g2816063622443.52512801.1bHLH29/FIT1
    At5g06490431293.046531.1C3HC4 RING zinc finger protein-like
    At2g20030892613.010697−1.1Putative RING zinc finger protein
Signaling
    At1g3476012810978.4153131−1.214-3-3 Protein
    At3g614101977103.6267247−1.1Putative protein protein kinase
    At2g19410441373.148501.0Putative protein kinase, Class 1
    At1g7728037511253.03764271.1Receptor-like protein kinase, Class 1
    At1g518603088472.8329206−1.6Receptor-like protein kinase, Class 1
    At5g355804129832.3431329−1.3Ser/Thr protein kinase-like, Class 1
    At5g010601302782.19179−1.2Putative protein kinase, Class 1
Metabolism
    At4g3194032172755.022241.1Cytochrome P450-like monooxygenase
    At3g532802751919.427271.0Cytochrome P450 71B5
    At5g02780222424519.12274151.8Putative protein In2
    At3g1290096173018.57166−1.1Hypothetical, similar to oxidoreductases
    At3g117504143310.544561.3Putative dihydroneopterin aldolase
    At4g02330643986.350501.0Hypothetical, similar to pectinesterase
    At5g3689046827846.2373369−1.0β-Glucosidase
    At4g31950251184.723241.0Cytochrome P450-like monooxygenase
    At2g0188026810414.03583851.1Putative purple acid phosphatase
    At2g02310451713.94945−1.1Putative phloem-specific lectin
    At1g097901174413.81151311.1Putative phytochelatin synthetase
    At3g470402479343.73073291.1β-d-Glucan exohydrolase-like protein
    At5g04950142549513.51147980−1.2NAS1, nicotianamine synthase
    At3g474202237403.3307145−2.1Putative sn-glycerol-3-phosphate permease
    At3g21240156246463.0178921421.2Putative 4-coumarate:CoA ligase 2
    At1g1891066318412.86987161.0Similar to flavonol-induced pollen germ.
    At1g6061037110252.83614931.4Similar to S-ribonuclease binding protein
    At4g2922075420272.7871840−1.0Phosphofructo-1-kinase-like
    At3g314151644192.6178168−1.1Vetispiradiene synthase, putative
    At2g0583069817612.5693666−1.0Putative translation initiation factor eIF-2B
    At2g30670741812.457741.3Putative tropinone reductase
    At2g400004149912.44284681.1Putative nematode-resistance protein
    At1g05530571332.35350−1.1Indole-3-acetate β-d-glucosyltransferase
    At4g129102064832.3250236−1.1Ser carboxypeptidase I precursor-like
    At3g5458087620492.31084592−1.8Extensin precursor-like protein
    At2g37040208942722.121452021−1.1Phe ammonia lyase (PAL1)
    At4g389501192432.1117115−1.0Kinesin-like protein
    At4g146802885952.1243222−1.1    ATP-sulfurylase
    At4g10510751542.159721.2Subtilisin-like Ser protease
Unknown
    At3g6193069222932.55554−1.0Hypothetical protein
    At4g1937011711579.71481991.3Hypothetical protein
    At3g58060686058.974771.0Putative protein
    At3g51200372747.434431.3Putative protein
    At1g4982060321603.67437611.0Unknown protein
    At5g54790762613.49772−1.3Unknown protein
    At3g484502747902.93073751.2Hypothetical protein
    At3g068902617132.82412651.1Hypothetical protein
    At5g4059077719582.5804577−1.4Putative protein
    At3g185603939072.3473346−1.4Unknown protein
    At2g4674082218252.2840768−1.1Unknown protein
Iron Regulation Is Partially Dependent on FIT1
Transporter
    At4g19680103215621.01493352.3IRT2, Fe(II) transport protein
    At3g469001337665.81412611.9COPT2, copper transport protein
Transcription Factor
    At1g5616075175623.4925826.3MYB72
    At3g128207079011.7942222.4MYB10
Signaling
    At1g057002326873.12313171.4Putative light repressible receptor kinase,
Metabolism
    At1g141901156395.61342511.9Putative mandelonitrile lyase
    At5g4791073327633.878510911.4RbohD, respiratory burst oxidase protein
Unknown
    At1g73120148154810.5783915.0Hypothetical protein
Baseline Expression Levels Higher in fit1-1 +Fe versus Wild-Type +Fe
Transporter
    At4g19690368614216.3122425162.1IRT1, Fe(II) transport protein
    At3g58810355425711.9108018641.7MTPa2, CDF family
    At5g0357077850976.6144120411.4FERROPORTIN2, putative Fe transporter
Metabolism
    At3g5074043738718.67819071.2UTP-glucose glucosyltransferase-like
Unknown
    At3g077209041166612.9352540781.2Unknown protein
  • The locus identifiers are given for genes that are at least twofold upregulated in response to iron deficiency in the wild type and are also at least twofold downregulated in fit1-1 iron-deficient roots compared to wild-type iron-deficient roots. Seventy-two genes met these two requirements and were found to be statistically significant. Wild-type and fit1-1 chip signal intensities and fold changes are presented for the average of two biological replicates. Genes are grouped based on their regulation by FIT1 and secondly by predicted function. Genes known to be involved in metal transport or homeostasis are shown in bold.