Table 1.

Concentration of Ascorbate and Glutathione in the Incubation Medium of H. vulgare Aleurone Layers as Determined by Enzyme-Coupled Assays and HPLC

Concentration (μM)Method
TimeABA-TreatedGA-TreatedEnzyme AssayECD
Total ascorbate21 h0.0 ± 0.00.0 ± 0.0*
48 h0.0 ± 0.00.0 ± 0.0*
Reduced ascorbate24 h0.0 ± 0.00.0 ± 0.0*
Total glutathione24 h0.8 ± 0.00.8 ± 0.1*
48 h0.1 ± 0.10.5 ± 0.2*
GSH24 h0.0 ± 0.00.5 ± 0.2*
GSSH24 h0.5 ± 0.60.7 ± 0.5*
  • Layers were incubated with either ABA or GA for the times indicated. The amounts of total ascorbate (reduced plus oxidized) and total glutathione were determined using enzyme-coupled assays. The amounts of reduced ascorbate, reduced glutathione (GSH), and fully oxidized glutathione (GSSH) were determined using an HPLC system equipped with an electrochemical detector (ECD). Each value is the mean ±sd of four flasks of aleurone layers.