Table 1.

GA 2-Oxidase Enzyme Activity Assay of the Recombinant AtGA2ox6 Protein

SubstrateProductMass Spectra of Products, Mass-to-Charge Ratio (% Relative Abundance)
17,17-2H2-GA12H2-GA8596(100), 581(6), 567(17), 537 (20), 450(24), 377 (10), 240(6)
17,17-2H2-GA42H2-GA34508(100), 418(16), 315(10), 290(6), 231(13), 225(16)
2H2-GA8 standard596(100), 581(5), 567(12), 537(13), 450(52), 377 (14), 240(12)
2H2-GA34 standard508(100), 418(22), 315(12), 290(11), 231(14), 225(34)
  • Products were identified by GC-MS after incubation of the substrate with recombinant MAL-AtGAox6 protein and conversion to Me-TMsi derivatives.