Table 2.

Fatty Acid Hydroxide Analysis of Germinating Seedlings

Genotype13-HODE cis/trans13-HODE trans/trans13-HOTE9-HODE cis/trans9-HODE trans/transΣLOH
0 d
    ColNDaNDNDNDNDND
    vte1-1NDNDNDNDNDND
    vte2-10.4 ± 0.30.3 ± 0.3ND0 ± 0.30 ± 0.20.3 ± 0.5
    WsNDNDNDNDNDND
    vte2-21.4 ± 0.61.8 ± 0.9ND1.0 ± 0.50.8 ± 0.52.6 ± 1.3
3 d
    Col0.4 ± 0.20.6 ± 0.3ND0.3 ± 0.2ND0.4 ± 0.1
    vte1-10.6 ± 0.10.3 ± 0.20 ± 0.10.3 ± 0.10 ± 0.10.4 ± 0.2
    vte2-127.7 ± 2.7 (53:47)34.5 ± 5.42.2 ± 0.8 (56:44)23.0 ± 0.1 (50:50)20.1 ± 2.448.3 ± 6.0
    Ws0.5 ± 0.2NDNDNDND0.1 ± 0.1
    vte2-225.6 ± 1.3 (52:48)30.0 ± 2.51.3 ± 0.5 (60:40)20.5 ± 1.6 (51:49)16.7 ± 1.146.6 ± 3.8
6 d
    Col1.2 ± 0.2 (51:49)1.0 ± 0.70.1 ± 0.10.6 ± 0.4 (52:48)0.6 ± 0.40.8 ± 0.4
    vte1-10.8 ± 0.1 (54:46)0.9 ± 0.2ND0.6 ± 0.1 (50:50)0.3 ± 0.20.7 ± 0.1
    vte2-131.3 ± 6.3 (52:48)30.0 ± 13.90.5 ± 0.9 (55:45)28.0 ± 4.6 (51:49)24.9 ± 9.242.6 ± 13.2
    Ws0.3 ± 0.1 (52:48)0.4 ± 0.20.0 ± 0.10.1 ± 0.2 (47:53)0.3 ± 0.20.2 ± 0.1
    vte2-230.8 ± 3.9 (52:48)27.1 ± 6.00.3 ± 0.3 (56:44)26.1 ± 3.1 (51:49)19.8 ± 1.342.1 ± 6.2
  • The five prevalent hydroxy fatty acid species were quantified using normal phase HPLC. Each compound is expressed as pmol/nmol of the corresponding PUFA (linoleic or linolenic acid) from which it was derived in the sample. The enantiomeric composition of 9-HODE cis/trans, 13-HOTE, and 13-HODE cis/trans were determined by chiral phase HPLC. The ratio of R to S is given in parentheses for samples that contained sufficient quantities for chiral analysis. Chiral analysis indicated that both 9- and 13-HODE trans/trans peaks contained two enantiomers in relatively equal proportions, though R and S could not be assigned because of the lack of commercially available standards. ΣLOH is sum of all LOHs expressed as pmol/nmol total PUFAs (linoleic and linolenic acid). The zero day time point is seed that have been imbibed for 5 d at 4°C. The data are the means ± sd (n = 4). The LOH values for both vte2-1 and vte2-2 were highly significant (Student's t test, P < 0.01) at all points relative to the wild type.

  • a ND, not detected.