Table 1.

Kinetin Induction of the Invertase Inhibitor P17A Results in Inhibition of Sucrose Cleavage

LineTimeTreatmentGlucose (mM/gFW)Fructose (mM/gFW)Sucrose (mM/gFW)
W381 dNone19.2 (2.4)13.5 (1.6)6.7 (1.2)
Kinetin19.7 (2.9)18.5 (2.8)9.1 (1.7)
3 dNone42.5 (0.4)41.3 (1.2)9.0 (4.4)
Kinetin47.5 (1.7)46.7 (1.7)9.1 (2.4)
Lin6:P17A1 dNone14.0 (6.1)11.6 (5.8)8.1 (2.1)
Kinetin6.0 (1.3)4.2 (1.6)6.6 (1.9)
3 dNone46.5 (3.5)33.9 (2.4)8.8 (0.1)
Kinetin19.9 (5.6)20.1 (5.4)9.1 (2.6)
  • Detached leaves of a transgenic tobacco line expressing the tobacco invertase inhibitor P17A under control of the cytokinin-inducible promoter Lin6 (Lin6:P17A) and wild-type plants (W38) were infiltrated with water containing kinetin at a final concentration of 30 μg/L, and concentrations of soluble sugars have been determined. The values represent the mean value of three independent replications ± se obtained with line NT71-29. Similar results have been obtained with line NT71-9.