Table 2.

Subcellular Localization of Selected PPR Proteins

AGI Code	PPR Subclass	Electroporation Experiments	Agroinfiltration Experiments	Organellar Proteomics	TargetP Prediction	Predotar Prediction
At1g11630	PPR	ND	ND	Mitochondria^a	Mitochondria	Mitochondria
At1g26460	PPR	ND	ND	Mitochondria^a	Mitochondria	Mitochondria
At1g55890	PPR	ND	ND	Mitochondria^a	Mitochondria	Possibly mitochondria
At1g59720	DYW	ND	Mitochondria^b ^c		Plastid	None
At1g60770	PPR	ND	ND	Mitochondria^a	None	Possibly mitochondria
At1g61870	PPR	Mitochondria^c ^d ^e	Mitochondria^c	Mitochondria^a	Mitochondria	Mitochondria
At2g01390	PPR	Mitochondria^c	ND		Plastid	Possibly mitochondria
At2g19280	PPR	Mitochondria^b	ND		Mitochondria	Possibly plastid
At2g20540	E+	No signal^d	Mitochondria^b		None	None
At2g34370	DYW	Mitochondria^c ^e	ND		Mitochondria	Mitochondria
At3g02010	DYW	Mitochondria^b ^d ^e	ND		Mitochondria	None
At3g11460	DYW	Mitochondria^d ^e	Mitochondria^b		Plastid	Plastid
At3g13160	PPR	ND	ND	Mitochondria^a	Mitochondria	Mitochondria
At3g13770	DYW	Mitochondria^b	No signal^c		Mitochondria	Mitochondria
At3g13880	E+	Mitochondria^b	No signal^c		Mitochondria	Possibly mitochondria
At3g24000	DYW	Mitochondria^d	Mitochondria^b		Mitochondria	Mitochondria
At3g26780	DYW	ND	ND	Mitochondria^a	Mitochondria	Mitochondria
At3g60960	PPR	ND	ND	Mitochondria^a	Mitochondria	Possibly mitochondria
At4g02750	DYW	Mitochondria^b	ND		Mitochondria	None
At4g20090	PPR	ND	Mitochondria^b		Plastid	Possibly ER
At4g35850	PPR	ND	ND	Mitochondria^a	Mitochondria	Mitochondria
At4g36680	PPR	ND	ND	Mitochondria^a	Mitochondria	Mitochondria
At5g09950	DYW	ND	Mitochondria^c		Mitochondria	Plastid
At5g13230	DYW	Mitochondria^b	ND		Mitochondria	Mitochondria
At5g14770	PPR	Mitochondria^b	No signal^c		Mitochondria	Mitochondria
At5g15340	DYW	Mitochondria^b	ND		Mitochondria	Mitochondria
At5g55740	E+	Mitochondria^b	Mitochondria^b		Plastid	Plastid
At1g74850	PPR	ND	ND	Plastid^f	Plastid	Possibly plastid
At2g01860	PPR	Plastids^b	ND		Plastid	Possibly plastid
At2g03880	DYW	Plastids^b	ND		Mitochondria	Mitochondria
At2g29760	DYW	Plastids^b	ND		Plastid	Plastid
At3g04760	PPR	ND	ND	Plastid^f	Plastid	Possibly plastid
At3g23020	PPR	ND	ND	Plastid^f	None	Possibly plastid
At3g42630	PPR	ND	ND	Plastid^f	None	Possibly mitochondria
At3g46870	PPR	ND	ND	Plastid^f	Plastid	Possibly mitochondria
At4g16390	PPR	Plastids^d ^e	ND		Plastid	Possibly plastid
At4g31850	PPR	Plastids^c ^e	ND		Plastid	Plastid
At5g13270	DYW	Plastids^b	ND		Plastid	Plastid
At5g52630	DYW	Plastids^c ^e	Plastids^b		None	None

↵a Organellar proteomics data from Heazlewood et al. (2004).
Localization was studied by fusion of presequences to the following:
↵b DsRed2,
↵c GFP, or
↵d full-length ORFs to DsRed2.
↵e The sequence of the entry clone used for the LR reaction was verified.
↵f Organellar proteomics data from Kleffmann et al. (2004).
Experimentally proven localizations were compared to bioinformatics predictions from TargetP and Predotar. The first and second parts of the table correspond to mitochondrial- and plastid-localized PPR proteins, respectively.