Table 2.

Subcellular Localization of Selected PPR Proteins

AGI CodePPR SubclassElectroporation ExperimentsAgroinfiltration ExperimentsOrganellar ProteomicsTargetP PredictionPredotar Prediction
At1g11630PPRNDNDMitochondriaaMitochondriaMitochondria
At1g26460PPRNDNDMitochondriaaMitochondriaMitochondria
At1g55890PPRNDNDMitochondriaaMitochondriaPossibly mitochondria
At1g59720DYWNDMitochondriabcPlastidNone
At1g60770PPRNDNDMitochondriaaNonePossibly mitochondria
At1g61870PPRMitochondriacdeMitochondriacMitochondriaaMitochondriaMitochondria
At2g01390PPRMitochondriacNDPlastidPossibly mitochondria
At2g19280PPRMitochondriabNDMitochondriaPossibly plastid
At2g20540E+No signaldMitochondriabNoneNone
At2g34370DYWMitochondriaceNDMitochondriaMitochondria
At3g02010DYWMitochondriabdeNDMitochondriaNone
At3g11460DYWMitochondriadeMitochondriabPlastidPlastid
At3g13160PPRNDNDMitochondriaaMitochondriaMitochondria
At3g13770DYWMitochondriabNo signalcMitochondriaMitochondria
At3g13880E+MitochondriabNo signalcMitochondriaPossibly mitochondria
At3g24000DYWMitochondriadMitochondriabMitochondriaMitochondria
At3g26780DYWNDNDMitochondriaaMitochondriaMitochondria
At3g60960PPRNDNDMitochondriaaMitochondriaPossibly mitochondria
At4g02750DYWMitochondriabNDMitochondriaNone
At4g20090PPRNDMitochondriabPlastidPossibly ER
At4g35850PPRNDNDMitochondriaaMitochondriaMitochondria
At4g36680PPRNDNDMitochondriaaMitochondriaMitochondria
At5g09950DYWNDMitochondriacMitochondriaPlastid
At5g13230DYWMitochondriabNDMitochondriaMitochondria
At5g14770PPRMitochondriabNo signalcMitochondriaMitochondria
At5g15340DYWMitochondriabNDMitochondriaMitochondria
At5g55740E+MitochondriabMitochondriabPlastidPlastid
At1g74850PPRNDNDPlastidfPlastidPossibly plastid
At2g01860PPRPlastidsbNDPlastidPossibly plastid
At2g03880DYWPlastidsbNDMitochondriaMitochondria
At2g29760DYWPlastidsbNDPlastidPlastid
At3g04760PPRNDNDPlastidfPlastidPossibly plastid
At3g23020PPRNDNDPlastidfNonePossibly plastid
At3g42630PPRNDNDPlastidfNonePossibly mitochondria
At3g46870PPRNDNDPlastidfPlastidPossibly mitochondria
At4g16390PPRPlastidsdeNDPlastidPossibly plastid
At4g31850PPRPlastidsceNDPlastidPlastid
At5g13270DYWPlastidsbNDPlastidPlastid
At5g52630DYWPlastidscePlastidsbNoneNone
  • a Organellar proteomics data from Heazlewood et al. (2004).

  • Localization was studied by fusion of presequences to the following:

  • b DsRed2,

  • c GFP, or

  • d full-length ORFs to DsRed2.

  • e The sequence of the entry clone used for the LR reaction was verified.

  • f Organellar proteomics data from Kleffmann et al. (2004).

    Experimentally proven localizations were compared to bioinformatics predictions from TargetP and Predotar. The first and second parts of the table correspond to mitochondrial- and plastid-localized PPR proteins, respectively.