Table 4.

List of Arabidopsis T-DNA Mutants with Insertions in PPR Genes

Mutant IDAGI CodeSubclassTargetingHomozygotesPhenotype of Homozygotes
DNR2At1g14470PLS?YesDwarf, reduced fertility
EAN42At1g20230DYW?Noa
Salk_033409At1g20230DYW?YesDwarf, late flowering
DVM10At2g40720E+?Yes
DDU146At3g25970E+?YesDwarf, reduced fertility
Salk_127777At3g25970E+?YesDwarf, reduced fertility
EMB 2744At5g39680DYW?NoEmbryo defective
DJU9At1g06710PPRMitochondriaYes
T243At1g22830EMitochondriaYes
DYK12At1g51965PPRMitochondriaYes
ABP1At1g80550PPRMitochondriaNo
Salk_024306At1g80550PPRMitochondriaNo
DNP1At2g20540E+MitochondriaYes
DXZ45At3g13150PPRMitochondriaYes
Salk_015469At3g49140DYWMitochondriaYes
EMB 1796At3g49240PPRMitochondriaNoEmbryo defective
EMB 1025At4g20090PPRMitochondriaNoEmbryo defective
EMB 2745At5g39710PPRMitochondriaNoEmbryo defective
Salk_033891At5g46460DYWMitochondriaYes
DSN8At5g62370PPRMitochondriaNo
DZY11At5g65570DYWMitochondriaYes
EMB 1444At1g06150E+PlastidsNoEmbryo defective
Salk_067029At1g09410DYWPlastidsYes
Gabi_104E05At1g09410DYWPlastidsYes
EMB 2279At1g30610PPRPlastidsNoEmbryo defective
CYK8At2g01860PPRPlastidsNoEmbryo defective
hcf152At3g09650PPRPlastidsYesDefective processing of petB
EMB 1270At3g18110PPRPlastidsNoEmbryo defective
Salk_040629At3g22150E+PlastidsYes
crr2At3g46790DYWPlastidsYesDefective processing of ndhB
Salk_046131At3g46790DYWPlastidsYesDefective processing of ndhB
Gabi_425F06At3g46790DYWPlastidsYesDefective processing of ndhB
EMB 2261At3g49170DYWPlastidsNoEmbryo defective
pgr3At4g31850PPRPlastidsYesCytochrome b6f and/or NDH-deficient
CRI6At4g31850PPRPlastidsYesYellow leaves
EMB 2453At4g39620PPRPlastidsNoEmbryo defective
EMB 1899At5g03800DYWPlastidsNoEmbryo defective
DYH216At5g27270PPRPlastidsNoEmbryo defective
EMB 1006At5g50280PPRPlastidsNoEmbryo defective
  • a Not determined.

  • The mutants are named according to the nomenclature of their collections of origin: Salk_xxx mutants are from the Salk collection (Alonso et al., 2003), Gabi_xxx mutants are from the GABI-Kat collection (Li et al., 2003), EMB xxx mutants are from the SeedGenes project (Tzafrir et al., 2003), and the other mutants are from the Versailles collection (Samson et al., 2002). The targeting column indicates predictions based on Predotar and TargetP scores (see Supplemental Table S3 online), or if the protein localization has been experimentally determined (Table 2), the organelle is indicated in bold. For each mutant, we indicate whether or not homozygotes could be obtained, and if so, the phenotype of the homozygous plants. When homozygotes could not be obtained, in some cases the siliques of heterozygous plants were examined for evidence of embryo abortion, and the phenotype is indicated as embryo defective. Several alleles of the mutants hcf152, crr2, and pgr3 have been described (Hashimoto et al., 2003; Meierhoff et al., 2003; Yamazaki et al., 2004). Unlike the other mutants listed here, these are mostly point mutations and are included for comparison.