Table 1.

Conservation of Phosphorylation Sites in Membrane Transporters

PlantsTFamilySubfamily/NameProteinPhosphorylation SiteConservation within Protein FamilyConservation among Putative Orthologs (EST Database)
2.A.1STPMonosaccharide transporterSTP1RGVDDVpSQEFDDL(+)
STP13FVNGEKpSNGKSNG+
PHTPhosphate transporterPHT1-4NEDNENpSNNDSRTn.f.
2.A.2SUCSucrose-proton symporterSUC5AANNApTALEpTQpSpSPEDLGQ3 (9)+
2.A.7PUPPurine permeasePUP18EPDQILpSPRRSLE2 (13)+
2.A.16TDTC4-dicarboxylate/malic acid transporter (telluride resistance)At4g13800PVFINpSGpSSRSSNS2 (7)n.f.
At5g24030DQLRNVpSpSENIENYLKn.f.
2.A.17POTProton-dependent oligopeptide transporterAt3g47960NTDVVDpSFEEEQR+
At1g69870GSFSKSpSPpSELDV..YKRIpSpSPGSILD(+)
2.A.18AAATamino acid/auxin permeaseAtLTH4PATPRVpSpTPEILTP+
At5g41800PVTRLDpSDAGALF(+)
At5g01240GRKVEDpSAAEEDI
2.A.29Mitochondrial carrierAt3g55640KFMYMVpTGMENHK+
2.A.31AEAnion exchangers/boron transporterAt3g06450EAPAILpSFNLKPE+
2.A.36NHXSodium-proton exchangerSOS1/NHX7VNVYRRpSVSFGGI+
NHX1,2VPFVPGpSPpTERNPPD+
2.A.40NCS2Nucleobase:cation symporter 2At2g27810GFRPKFpSGEpTTApTDpSpSSGQLSL+
2.A.49AMTAmmonium transporterAMT1MAGMDMpTRHGGFA3 (6)+
2.A.55NRAMPMetal ion (Fe/Mn) transporterNRAMP1QLPCRVpSTSDVD+
2.A.57ENTEquilibrative nucleoside transporterENT6AGIQNQpSDLpSDDDSKN4 (7)+
2.A.72KUPK+ uptake permeaseKUP2EDDNARpSVQpSNEpSSSESR(+)
KUP11DQKLDQpSMDEEAG
  • The conservation of a phosphorylatable residue (S or T) in paralogs and putative orthologs of phosphorylated transporters was analyzed. Phosphoproteins were grouped into families according to PlantsT (http://plantst.sdsc.edu/). Proteins listed in the Aramemnon database (http://aramemnon.botanik.uni-koeln.de/) as isospecific homologs of the phosphoprotein with at least 30% similarity were called protein family. Under the heading Conservation within Protein Family, the number of family members that share the phosphorylation site is listed. Under the heading Conservation among Putative Orthologs, (+) denotes one BLAST hit in the EST database with conserved phosphorylation site; + denotes two or more hits; n.f., no ambiguous ortholog was found in the EST databases.