Table 4.

Glycosyl Compositions of Fractions from Wild-Type and fra8 Cell Walls

Cell Wall FractionRhaFucAraXylMan4-O-Me-GlcAGalGlcGalAGlcATotal
PectinWT7.940.878.360.870.250.5910.590.3411.230.9742.02
fra811.091.1112.131.070.330.9015.920.5315.261.1259.47
XEGWT00.220.041.170.05nd0.652.05ndnd4.19
fra800.380.071.890.16nd1.103.55ndnd7.14
1 N KOHWT1.040.301.3958.510.385.001.833.271.893.2576.86
fra81.380.922.7515.491.372.374.809.163.550.6042.40
4 N KOHWT0.830.781.3337.972.172.552.756.222.881.7359.21
fra81.011.762.0619.265.742.924.2010.124.680.3452.09
TFAWT1.811.102.3020.235.784.057.4514.566.472.6266.37
fra81.740.722.7615.806.643.045.4710.028.461.0455.69
CelluloseWT331.65
fra8243.45
TotalWT11.623.2813.42118.758.6412.1823.29358.0922.468.57580.29
fra815.224.8919.7653.5014.249.2431.50276.8331.963.10460.23
  • Data are means (mg of sugar/g of AIR) of two duplications of two independent reductions. AIR was fractioned sequentially with a combination of EPG and pectin methylesterase (PME) (pectin fraction), a xyloglucan-specific endoglucanase (XEG fraction), and 1 and 4 N KOH (KOH fractions). The resulting residue was hydrolyzed with TFA (TFA fraction) or with sulfuric acid to determine cellulose content (see Methods). Uronosyl residues in the fraction were reduced to their correspondent 6-6 dideutered sugars before sugar analysis (as alditol acetates). The 3-0-methyl glucose (Biochemical) was used as standard to determine the amount of 4-O-methyl glucuronic acid after reduction. Gas chromatography–mass spectrometry and gas chromatography with flame-ionization detection were used for identification and quantification of the monosaccharides. Bold numbers highlight significant changes between the wild type and fra8. For clarity, the variance is not shown but is <10%. nd, not determined.