Table 1.

Segregation of Seedling Phenotypes

Seedling Phenotypesa
Parental GenotypeBG/MediabnNormalNo SAMTwo RSCOne RSC
rev4XL/Soil18999.5 ± 1.0%0.5 ± 1.0%00
rev5XC/Soil14999.3 ± 1.4%0.7 ± 1.4%00
rev phb-11/+3XL/Soil20379.8 ± 5.6%12.8 ± 4.7%07.4 ± 3.7%
rev phb-13/+3XL/Soil19881.3 ± 5.5%10.6 ± 4.4%08.1 ± 3.9%
rev/+ phb-13c5XC/MS93581.7 ± 2.5%16.9 ± 2.5%01.4 ± 0.8%
rev/+ phb-13c5XC/Soil47687.6 ± 3.0%d12.4 ± 3.0%00
rev/+ phb-13 athb87XC/Soil30580.0 ± 4.6%d18.7 ± 4.5%01.3 ± 1.3%
rev phv3XL/Soil5896.6 ± 4.8%3.4 ± 4.8%00
rev phv5XC/Soil10499.0 ± 2.0%1.0 ± 2.0%00
rev/+ phb-11 phv3XL/MS87574.9 ± 2.9%2.9 ± 1.1%2.6 ± 1.1%19.7 ± 2.7%
rev/+ phb-11 phv2XC/MS22878.5 ± 5.4%0021.5 ± 5.4%
rev/+ phb-12 phv5XC/MS177776.8 ± 2.0%00.2 ± 0.2%23.0 ± 2.0%
rev/+ phb-13 phv5XC/MS105674.9 ± 2.7%0.1 ± 0.2%0.1 ± 0.2%24.9 ± 2.7%
rev/+ phb-13 phv athb87XC/MS30173.4 ± 5.1%00.3 ± 0.6%26.2 ± 5.1%
rev/+ phb-11 cna3XL/MS39576.5 ± 4.3%0.3 ± 0.6%7.3 ± 2.6%15.9 ± 3.7%
rev/+ phb-13 cna5XC/MS17474.1 ± 6.6%0.6 ± 1.2%025.3 ± 6.6%
rev/+ phb-13 phv cna5XC/MS96867.1 ± 3.0%9.6 ± 1.9%e1.4 ± 0.8%e21.8 ± 2.7%e
rev/+ phb phv cna athb8/+5XC/MS28868.8 ± 5.5%10.8 ± 3.7%f1.0 ± 1.2%19.4 ± 4.7%f
rev/+ phb/+ phv cna athb8g6XC/MS32082.8 ± 4.2%11.6 ± 3.6%g0.3 ± 0.6%g5.3 ± 2.5%g
  • a Progeny of plants of the given genotype were sown and the percentage in each phenotypic class is reported. Normal, meristem active at least until flowering; No SAM, meristem not formed or terminated before flowering; Two RSC, two radially symmetric cotyledons; One RSC, apical portion consists of a radially symmetric cotyledon. Numbers indicate percent of total ± 2*se of the proportion.

  • b BG, genetic background; numbers indicate the number of times the lines were introgressed into Col (C) or Ler (L). Seeds were sown either on soil or on sterile 0.5× MS media (MS).

  • c Two different batches of rev/+ phb-13 seeds were tested.

  • d Among the Normal seedlings, 66 rev/+ phb-13 (13.9%) and 13 rev/+ phb-13 athb8 (4.3%) plants had narrow-leaf and lateral-meristemless phenotypes and were found to be rev phb double mutants.

  • e Three of three meristemless progeny of the rev/+ phb phv cna plant were found to be rev/+ upon genotyping. One Two RSC and two One RSC seedlings were genotyped and found to be rev/rev.

  • f Three No SAM progeny of the rev/+ phb phv cna athb8-12/+ plant were found to be rev/+ upon genotyping (two of these were ATHB8+ and one was homozygous athb8-12). One Two-RSC seedling was genotyped and found to be rev phb phv cna athb8-12/+. Of five One RSC seedlings genotyped, three were rev phb phv cna athb8-12/+ and two were rev phb phv cna athb8-12.

  • g Two of three No SAM progeny of the rev/+ phb/+ phv cna athb8-11/athb8-12 plant were found to be rev phb/+ phv cna athb8 upon genotyping; the third meristemless plant was rev/+ phb phv cna athb8. Both One RSC seedlings analyzed were found to be quintuple mutants (one was athb8-12 and one was athb8-11/athb8-12).