Table 1.

Segregation of Seedling Phenotypes

			Seedling Phenotypes^a
Parental Genotype	BG/Media^b	n	Normal	No SAM	Two RSC	One RSC
rev	4XL/Soil	189	99.5 ± 1.0%	0.5 ± 1.0%	0	0
rev	5XC/Soil	149	99.3 ± 1.4%	0.7 ± 1.4%	0	0
rev phb-11/+	3XL/Soil	203	79.8 ± 5.6%	12.8 ± 4.7%	0	7.4 ± 3.7%
rev phb-13/+	3XL/Soil	198	81.3 ± 5.5%	10.6 ± 4.4%	0	8.1 ± 3.9%
rev/+ phb-13^c	5XC/MS	935	81.7 ± 2.5%	16.9 ± 2.5%	0	1.4 ± 0.8%
rev/+ phb-13^c	5XC/Soil	476	87.6 ± 3.0%^d	12.4 ± 3.0%	0	0
rev/+ phb-13 athb8	7XC/Soil	305	80.0 ± 4.6%^d	18.7 ± 4.5%	0	1.3 ± 1.3%
rev phv	3XL/Soil	58	96.6 ± 4.8%	3.4 ± 4.8%	0	0
rev phv	5XC/Soil	104	99.0 ± 2.0%	1.0 ± 2.0%	0	0
rev/+ phb-11 phv	3XL/MS	875	74.9 ± 2.9%	2.9 ± 1.1%	2.6 ± 1.1%	19.7 ± 2.7%
rev/+ phb-11 phv	2XC/MS	228	78.5 ± 5.4%	0	0	21.5 ± 5.4%
rev/+ phb-12 phv	5XC/MS	1777	76.8 ± 2.0%	0	0.2 ± 0.2%	23.0 ± 2.0%
rev/+ phb-13 phv	5XC/MS	1056	74.9 ± 2.7%	0.1 ± 0.2%	0.1 ± 0.2%	24.9 ± 2.7%
rev/+ phb-13 phv athb8	7XC/MS	301	73.4 ± 5.1%	0	0.3 ± 0.6%	26.2 ± 5.1%
rev/+ phb-11 cna	3XL/MS	395	76.5 ± 4.3%	0.3 ± 0.6%	7.3 ± 2.6%	15.9 ± 3.7%
rev/+ phb-13 cna	5XC/MS	174	74.1 ± 6.6%	0.6 ± 1.2%	0	25.3 ± 6.6%
rev/+ phb-13 phv cna	5XC/MS	968	67.1 ± 3.0%	9.6 ± 1.9%^e	1.4 ± 0.8%^e	21.8 ± 2.7%^e
rev/+ phb phv cna athb8/+	5XC/MS	288	68.8 ± 5.5%	10.8 ± 3.7%^f	1.0 ± 1.2%	19.4 ± 4.7%^f
rev/+ phb/+ phv cna athb8^g	6XC/MS	320	82.8 ± 4.2%	11.6 ± 3.6%^g	0.3 ± 0.6%^g	5.3 ± 2.5%^g

↵a Progeny of plants of the given genotype were sown and the percentage in each phenotypic class is reported. Normal, meristem active at least until flowering; No SAM, meristem not formed or terminated before flowering; Two RSC, two radially symmetric cotyledons; One RSC, apical portion consists of a radially symmetric cotyledon. Numbers indicate percent of total ± 2*se of the proportion.
↵b BG, genetic background; numbers indicate the number of times the lines were introgressed into Col (C) or Ler (L). Seeds were sown either on soil or on sterile 0.5× MS media (MS).
↵c Two different batches of rev/+ phb-13 seeds were tested.
↵d Among the Normal seedlings, 66 rev/+ phb-13 (13.9%) and 13 rev/+ phb-13 athb8 (4.3%) plants had narrow-leaf and lateral-meristemless phenotypes and were found to be rev phb double mutants.
↵e Three of three meristemless progeny of the rev/+ phb phv cna plant were found to be rev/+ upon genotyping. One Two RSC and two One RSC seedlings were genotyped and found to be rev/rev.
↵f Three No SAM progeny of the rev/+ phb phv cna athb8-12/+ plant were found to be rev/+ upon genotyping (two of these were ATHB8+ and one was homozygous athb8-12). One Two-RSC seedling was genotyped and found to be rev phb phv cna athb8-12/+. Of five One RSC seedlings genotyped, three were rev phb phv cna athb8-12/+ and two were rev phb phv cna athb8-12.
↵g Two of three No SAM progeny of the rev/+ phb/+ phv cna athb8-11/athb8-12 plant were found to be rev phb/+ phv cna athb8 upon genotyping; the third meristemless plant was rev/+ phb phv cna athb8. Both One RSC seedlings analyzed were found to be quintuple mutants (one was athb8-12 and one was athb8-11/athb8-12).