Table 3. Isomer Compositions of Strictosidinic Acid and Poststrictosidinic Acid Metabolites in Different Tissues of CYC1-RNAi Lines Compared to the Wild Type
MetaboliteIsomerWild TypeCYC1-RNAi
RootStemShoot ApexYoung LeafMature LeafRootStemShoot ApexYoung LeafMature Leaf
Strictosidinic acid1n.d.447 ± 147475 ± 87342 ± 54n.d.n.d.n.d.**130 ± 116**42 ± 58**n.d.
2997 ± 2983,970 ± 1,09845,252 ± 3,95840,524 ± 3,5402,808 ± 1,220155 ± 112**1,025 ± 822*3,436 ± 1,811**2,742 ± 884**2,070 ± 733
323,477 ± 5,21930,066 ± 5,28462,147 ± 4,21350,004 ± 2,8404,400 ± 1,3793,020 ± 1,826**1,209 ± 712**8,022 ± 4,086**2,381 ± 1,108**49 ± 67**
Strictosamide1936 ± 1751,511 ± 264249 ± 41n.d.n.d.n.d.**n.d.**n.d.**n.d.n.d.
2323 ± 65222 ± 24975 ± 1211,305 ± 1071,306 ± 170165 ± 74*21 ± 13**125 ± 35**132 ± 35**149 ± 127**
Pumiloside1219 ± 90520 ± 130n.d.n.d.n.d.n.d.**152 ± 90*n.d.n.d.n.d.
228 ± 11360 ± 251,329 ± 167928 ± 153179 ± 65n.d.**n.d.**127 ± 45**n.d.**n.d.**
Deoxypumiloside1373 ± 661,291 ± 141n.d.n.d.n.d.63 ± 61**372 ± 169**n.d.n.d.n.d.
2955 ± 2031,266 ± 395111 ± 88n.d.n.d.165 ± 85**436 ± 108*n.d.n.d.n.d.
  • Tissues were collected from plants grown under greenhouse cultivation for 8 months and metabolite levels determined by UHPLC/MS. Average levels expressed as response per kilogram fresh weight are shown with sd for biological replicates. For the wild type, three plants independently derived from tissue culture were analyzed and for CYC1-RNAi lines, and five plants from independently derived transgenic events were analyzed. Asterisks indicate significantly different metabolite levels in CYC1-RNAi lines (unpaired t test; *P < 0.05; **P < 0.001) relative to the wild type. n.d., below the limit of quantification, which is defined as signal/noise = 10.