Table 3.

Validation of Genes with Significant Differential Expression during Early Endosperm Formationa

GenBank EntryP ValuesbRatiocLog U.limdLog L.limePredicted FunctionRT-PCRf
AI8812930.00151.631.2810.135ATPase inhibitor
AI6499100.00013.032.1701.024Aconitate hydratase+
AI6650040.00010.42−0.671−1.839F-box protein+
AI8614940.00041.721.3550.209Galactosyltransferase+
AI4384130.00031.761.4000.233Helicase+
AI6295340.00200.63−0.087−1.233Importin
AI8203920.00071.701.3520.184γ-Zein mRNA+
AI7704500.00080.60−0.160−1.327Membrane protein+
AI8203860.00013.042.1771.031Metal binding protein+
AI7345430.00010.55−0.281−1.449GTP binding protein
AI8818780.00012.011.5940.427Receptor kinase
AI6227330.00031.751.3790.233Replication protein+
AI7149180.00012.631.9790.811Ribosomal protein+
AI4615640.00060.59−0.190−1.336RNA binding protein+
AI8203020.00111.661.3020.156sup1+
AI6770720.00010.19−1.794−2.962Tumor protein+
AI4615660.00410.64−0.066−1.234Unknown protein
AI6192470.00100.61−0.139−1.307Unknown protein+
AI6651510.00012.631.9790.812Unknown protein
AI7149000.00011.621.1090.290Unknown protein+
AI7150720.00121.671.3190.152Unknown protein+
AI7379790.00400.64−0.061−1.207Unknown protein
AI7709430.00301.571.2210.075Unknown protein+
AI8334770.00420.64−0.064−1.210Unknown protein+
AI8345980.00010.32−1.242−2.061Unknown protein+
AI8550490.00401.571.2340.066Unknown protein+
AI8614720.00401.541.1970.051Unknown protein+
  • a Twenty-seven genes identified from the microarray data were validated with semiquantitative RT-PCR.

  • b Individual P values obtained from the microarray data with the mixed linear model.

  • c Ratio calculated using the mean of the adjusted signal intensity values.

  • d Log value of the upper limit of the confidence interval.

  • e Log value of the lower limit of the confidence interval.

  • f The plus sign indicates data confirmed by semiquantitative RT-PCR (i.e., for which the microarray data and the RT-PCR indicate a change in the same direction when comparing mature unfertilized ovules and ovules 3 DAP); the minus sign indicates no significant difference detected by RT-PCR.