Table 1.

Primer Pair Combinations Used for Construction of Deletion Constructs

ConstructN-Terminal End Amplified with:C-Terminal End Amplified with:
pΔ39aaCR5/CREC5 (see text)CREC3 (see text)/CR3
pΔ39aa:GFPCR5/CREC5CREC3/GFP3STOP
pΔTNFRCR5/CRTN5 (5′-AGATCTATACAGAGTCCTGGTG-3′)CRTN3 (5′-GGATCCCAGTGGAGGCAAGGAAAAAG-3′)/CR3
pΔTNFR:GFPCR5/CRTN5CRTN3/GFP3STOP
pΔTM/K/C-terCR5/CRNM5 (5′-GGATCCTTTTTCCTTGCCTCCACTGG-3′)
pΔTM/K/C-ter:GFPCR5/CRNM5CRCT3 (5′-AGATCTAGAATGAGTAAAGGAGAAGAAC-3′)/GFP3STOP
pΔC-terCR5/CRCTstop (5′-GAGCTCTATAGCTGTGCAAGCGCTCG-3′)
pΔC-ter:GFPCR5/CRCT5 (5′-GGATCCCATTAGCTGTGCAAGC-3′)CRCT3/GFP3STOP