Table 3.

ABA-Repressed Genes in Guard Cells

Experiment 1Experiment 2Experiment 3
Affy ProbeaDescriptionAGI NumberbClustercRatiodP-ValueeFold ChangefRatioP-ValueFold ChangeRatioP-ValueFold ChangeSeki et al.gHoth et al.h
12100_atUnknown proteinAt2g21650V−3.80.99997−13.9−3.30.99999−9.8−3.40.99997−10.6
12169_i_atUnknown proteinAt4g33960V−0.70.50000−1.6−2.00.99952−4.0−1.60.99887−3.0
12185_atUnknown proteinAt1g54860V−1.80.95007−3.5−2.10.99898−4.3−1.70.99733−3.2
12196_atUnknown proteinAt2g28410V−1.51.00000−2.8−1.81.00000−3.5−2.00.99998−4.04.7
12284_atSomatic embryogenesis receptor kinaseAt1g34210V−0.80.96060−1.7−1.40.99977−2.6−2.00.99980−4.0
12338_atPectin methylesteraseAt3g14310V−4.10.99994−17.1−1.70.99977−3.2−2.30.97409−4.9
12381_atNo hitsV−0.60.94811−1.5−0.70.99733−1.6−1.50.99996−2.8
12409_atSmall nuclear ribonucleoprotein U1AAt2g47580V−0.81.00000−1.7−0.90.99992−1.9−2.41.00000−5.3
12768_atUnknown proteinAt2g15890V−0.90.99999−1.9−0.70.99995−1.6−1.60.99973−3.0
12773_atMembrane channel proteinAt2g28900V−0.91.00000−1.9−0.61.00000−1.5−1.20.99122−2.3
12843_s_atUnknown proteinAt2g16590V−1.00.99994−2.0−0.60.99947−1.5−0.80.50000−1.7
12846_s_atNo hitsV−2.11.00000−4.3−0.80.99999−1.7−1.51.00000−2.8
12881_s_atNo hitsV−1.60.99995−3.0−0.71.00000−1.6−2.31.00000−4.9
12893_atAlcohol dehydrogenase (EC 1.1.1.1) class IIIAt5g43940V−1.60.99989−3.0−0.60.99964−1.5−1.60.99998−3.0
13080_at40S ribosomal proteinAt2g21580V−0.70.99776−1.6−0.60.99905−1.5−1.81.00000−3.5
13184_s_atThioredoxinAt5g42980V−2.31.00000−4.9−0.61.00000−1.5−0.90.86906−1.9
13236_at2-Dehydro-3-deoxyphosphoheptonate aldolaseAt4g39980V−0.60.97627−1.5−1.20.99843−2.3−2.11.00000−4.3
13254_atFarnesyl pyrophosphate synthetase 2At4g17190V−0.70.99999−1.6−0.70.99973−1.6−1.81.00000−3.5
13554_atβ-Ketoacyl-CoA synthaseAt2g16280V−1.10.99853−2.1−1.61.00000−3.0−1.80.99776−3.5
13585_atUnknown proteinAt2g34170V−0.80.65272−1.7−0.90.99992−1.9−2.40.99999−5.3
13672_atNo hitsV−0.90.99967−1.9−1.00.99993−2.0−1.40.99939−2.6
14090_i_atUnknown proteinAt2g15830V−0.60.99820−1.5−0.61.00000−1.5−0.70.76103−1.6
14663_s_atGlycosyl hydrolase family 37 (trehalase)At4g24040V−1.00.99978−2.0−0.60.99910−1.5−0.60.49254−1.5
14667_atTrp synthaseAt5g54810V−0.70.99977−1.6−0.90.99967−1.9−2.30.99999−4.9
14717_s_atCLC-a chloride channel proteinAt5g40890V−0.70.99993−1.6−1.11.00000−2.1−2.61.00000−6.1
15194_s_atGASA4At5g15230IV−0.81.00000−1.7−0.91.00000−1.9−0.80.89823−1.7
15213_s_atOrn carbamoyltransferase precursorAt1g75330V−0.80.99991−1.7−0.91.00000−1.9−2.01.00000−4.0
15242_s_atPutative ACC oxidaseAt4g23340V−1.90.99984−3.7−1.50.99892−2.8−0.90.26247−1.9
15453_atUnknown proteinAt4g14020V−0.90.99999−1.9−0.70.99990−1.6−1.91.00000−3.7
15510_r_atNo hitsV−1.80.82023−3.5−0.80.99989−1.7−2.11.00000−4.3
15534_f_atHistone H4At2g28740V−0.80.99971−1.7−1.00.99994−2.0−2.31.00000−4.9
15548_atUnknown proteinAt1g11820V−0.90.99971−1.9−0.70.99978−1.6−2.31.00000−4.9
15624_s_atADPG pyrophosphorylase small subunitAt5g48300IV−0.60.99931−1.5−1.21.00000−2.3−2.21.00000−4.6
15626_s_atSpot 3 protein and vacuolar sorting receptor homologAt3g52850V−0.60.98817−1.5−0.80.99998−1.7−1.70.99999−3.2
15634_s_atNucleotide diphosphate kinaseAt5g63310V−2.80.99789−7.0−0.80.96925−1.7−1.70.99989−3.2
15703_i_atPutative trypsin inhibitorAt2g43520V−2.60.99971−6.1−0.81.00000−1.7−1.80.99934−3.5
16014_atUnknown proteinAt1g75750V−0.81.00000−1.7−0.81.00000−1.7−1.90.99999−3.7
16042_s_atCytochrome P450iAt5g05690V−0.70.99910−1.6−1.30.99986−2.5−0.70.54467−1.61.20
16051_atTubulin β-9 chainAt4g20890V−1.20.99997−2.3−0.90.99988−1.9−1.60.99990−3.0
16092_s_atPotassium channel protein KAT1At5g46240V−2.30.99981−4.9−1.50.99999−2.8−2.30.99999−4.9
16118_s_atPutative WD-40 repeat protein, MSI4At2g19520V−0.70.50000−1.6−0.90.99905−1.9−2.51.00000−5.7
16138_atSmall zinc finger–like proteinAt5g50810V−1.40.99748−2.6−0.70.96217−1.6−2.31.00000−4.9
16145_atVacuolar ATP synthaseAt1g75630V−1.01.00000−2.0−0.90.99996−1.9−2.90.99999−7.5
16416_atPlant defensin proteinAt2g02130V−0.80.99989−1.7−1.81.00000−3.5−0.90.92765−1.9
16430_atGlutathione S-transferasejAt2g30860V−0.80.99733−1.7−1.31.00000−2.5−2.01.00000−4.01.10
16451_atKetol-acid reductoisomeraseAt3g58610V−1.30.99420−2.5−1.51.00000−2.8−1.60.99999−3.0
16458_s_atNo hitsV−0.90.99993−1.9−0.70.99980−1.6−1.60.70618−3.0
16624_s_atTranscription factorAt1g08810V−0.60.84813−1.5−1.41.00000−2.6−1.90.99997−3.7
16897_i_atUnknown proteinAt5g15350V−0.70.99713−1.6−1.00.99992−2.0−2.30.99994−4.9
16985_s_atCytosolic ribosomal proteinAt3g48930V−0.71.00000−1.6−0.60.99999−1.5−1.90.99994−3.7
16998_atdTDP-glucose 4-6-dehydrataseAt3g62830V−0.70.99962−1.6−0.60.99985−1.5−1.80.99998−3.5
17033_atUnknown proteinAt5g39410V−1.00.99910−2.0−1.00.99999−2.0−1.40.90472−2.6
17126_atWD-40 repeat protein MSI1At5g58230V−0.90.82023−1.9−0.60.99992−1.5−2.11.00000−4.3
17187_atPutative arginaseAt4g08870V−3.20.99853−9.2−1.60.99763−3.0−1.40.99479−2.6
17215_atTrypsin inhibitorAt2g43550V−0.80.94609−1.7−1.00.99950−2.0−2.10.99991−4.3
17237_atUnknown proteinAt2g22840V−1.70.77836−3.2−2.30.99926−4.9−2.60.99973−6.1
17475_atSer/Thr-specific protein kinase ATPK64At5g55910V−1.90.99701−3.7−0.70.97295−1.6−1.50.99990−2.8
17865_atNADPH oxidoreductaseAt1g75280V−1.20.97295−2.3−2.70.99939−6.5−1.80.99984−3.5
17957_atAcetone-cyanohydrin lyaseAt2g23600IV−1.20.99910−2.3−3.31.00000−9.8−2.91.00000−7.5
18063_i_atNo hitsV−1.20.99425−2.3−1.00.99915−2.0−2.70.99997−6.5
18272_atUnknown proteinAt2g40080V−1.10.94402−2.1−3.91.00000−14.9−2.70.99999−6.5
18315_s_atβ-GlucosidaseAt4g27830V−0.70.99718−1.6−1.10.98873−2.1−2.30.99999−4.9
18365_s_atAuxin binding protein 1 precursorAt4g02980V−0.90.61045−1.9−1.21.00000−2.3−2.00.99987−4.0
18678_atPeptidylprolyl isomerase ROC4At3g62030V−1.80.99853−3.5−0.90.99995−1.9−2.51.00000−5.70.19
18687_atPyruvate dehydrogenase E1 α-subunitAt1g59900V−0.70.99973−1.6−0.81.00000−1.7−1.81.00000−3.5
18708_atRipening-related proteinAt5g62350V−1.40.99998−2.6−2.31.00000−4.9−2.81.00000−7.00.66
18882_atNo hitsV−0.70.99996−1.6−0.70.99943−1.6−1.81.00000−3.5
18956_atEthylene response sensorAt2g40940V−0.70.99943−1.6−0.60.99992−1.5−1.91.00000−3.7
19171_atPutative trypsin inhibitorkAt2g43510V−1.50.99967−2.8−0.60.99885−1.5−2.51.00000−5.72.97
20282_atProtein kinaseAt2g28930V−1.30.99989−2.5−1.31.00000−2.5−3.21.00000−9.2
20537_atExtensinAt4g13340V−1.60.99892−3.0−0.90.99733−1.9−1.20.93019−2.3
20585_atGlutaredoxinAt2g47880V−1.70.99994−3.2−1.90.94811−3.7−1.60.99950−3.0
20709_s_atPutative 3-oxoacyl (acyl-carrier protein) reductaseAt1g24360V−0.60.99998−1.5−0.60.99998−1.5−2.21.00000−4.6
  • a Describes names of probe set on Affymetrix chip.

  • b Arabidopsis Genome Initiative locus numbers.

  • c Indicates clusters in Figure 5.

  • d Signal log ratio, which measures the change in expression level for a transcript between two arrays. This change is expressed as the log2 ratio. A log2 ratio of 1 is the same as a fold change of 2.

  • e Change P-value, which measures the probability that the expression levels of a transcript in two different arrays are the same. Change P-values of 1.00000 correspond to P-value > 0.99995.

  • f Fold changes were calculated using the signal log ratio.

  • g Fold changes obtained from Supplemental Table 5 online (column for 5-h ABA treatment; Seki et al., 2002).

  • h Fold changes obtained from Supplemental Table Repressed.xls online (Hoth et al., 2002).

  • i Cytochrome p450 gene expression is repressed by ABA at 1, 2, 10, and 24 h of ABA treatment but shows ABA induction at 5 h of ABA treatment (see Seki et al., 2002, Supplemental Table 5 online).

  • j GST gene expression is slightly induced by ABA at 1, 5, and 10 h of ABA treatment but repressed by ABA at 2 and 24 h of ABA treatment (see Seki et al., 2002, Supplemental Table 5 online).

  • k Trypsin inhibitor gene is induced by ABA (see Seki et al., 2002, Supplemental Table 1 online).

  • This table contains genes that have a signal log ratio ≤ −0.6 in the three independent comparison analyses and a change P-value ≥ 0.997 in at least two independent comparison analyses (decrease or marginal decrease in Affymetrix nomenclature). A third comparison analysis with a change call of no change corresponding to a detection P-value < 0.997 was allowed.