Table 1.

Microtubule Dynamic Instability Parameters of L. japonicus Root Hair Cells

ParametersZone IZone IIZone III
Control plants
Rate (μm/min)
    Growth4.62 ± 0.164.48 ± 0.383.11 ± 0.12**
    Disassembly6.84 ± 0.726.62 ± 0.956.31 ± 0.31ns
Transition frequencies (events/min)
    Rescue8.15 ± 2.717.83 ± 2.112.39 ± 0.22**
    Catastrophe2.14 ± 0.201.85 ± 0.141.38 ± 0.17*
Plants inoculated with M. loti
Rate (μm/min)
    Growth2.50 ± 0.07**2.41 ± 0.20**2.49 ± 0.13*
    Disassembly6.27 ± 0.62ns6.22 ± 0.46ns6.11 ± 0.34ns
Transition frequencies (events/min)
    Rescue3.53 ± 1.10**3.32 ± 0.63**1.71 ± 0.18*
    Catastrophe1.42 ± 0.12*1.30 ± 0.10*0.96 ± 0.23ns
Plants inoculated with Nod factors
Rate (μm/min)
    Growth2.32 ± 0.12**2.19 ± 0.10**2.33 ± 0.12*
    Disassembly6.23 ± 0.70ns6.16 ± 0.53ns6.08 ± 0.34ns
Transition frequencies (events/min)
    Rescue3.33 ± 1.22**3.08 ± 0.51**1.10 ± 0.12**
    Catastrophe1.30 ± 0.10*1.14 ± 0.14*0.83 ± 0.19*
  • **, Significant at P < 0.001, according to ANOVA; *, significant at P < 0.05, according to ANOVA; ns, not significant at P = 0.05. Values for control Zone III root hairs were compared with those for Zone I and Zone II. Values after inoculation with M. loti TONO were determined from images acquired 24 to 48 h after plant inoculation and were compared with those for controls. Values after application of M. loti Nod factors were determined from images acquired 2 to 12 h after plant inoculation and were compared with those for controls. All values represent the means ± SE.