Table 1.

Summary of T1 Plant Expression, Rhythmicity, and Phase Information

Independent TransgenicsExpressionaRhythmic PlantsbPhase (CT)c
ConstructnVisible%Average CountsseP Valuedn%Averagesd
generic_EE1196151.3335.061.95691.813.50.44
generic_EEmt342367.63069.2626.52.8e-528.7N.D.N.D.
CCR2_EE847083.3745.9204.86998.613.40.31
CCR2_EEmt441431.882.822.37.0e-8535.7N.D.N.D.
PRR9_EE716490.1185.327.76195.312.80.51
PRR9_EEmt432455.8138.477.1N.D.2187.51.60.57
PRR9_EEmt432455.8138.477.12187.51.60.57
ME_scan11031312.624.43.70.006323.1N.D.N.D.
ME_scan233618.223.27.38.6e-6233.3N.D.N.D.
ME_scan33425.917.14.36.8e-8150.0N.D.N.D.
ME_scan4413995.1973.0122.21.2e-103384.65.01.92
ME_scan5917784.6133.512.4N.D.4355.82.51.57
PRR9_EE716490.1185.327.76195.312.80.51
EE_scan1827389.0418.387.30.37298.613.40.30
EE_scan_2997373.789.712.81.5e-46893.212.40.23
EE_scan_3382771.11960.3555.60.005414.8N.D.N.D.
EE_scan_4413995.11057.7138.53.6e-101846.214.21.88
EE_scan_51392719.433.611.61.3e-151348.112.91.12
EE_scan_6635384.181.99.90.015196.213.10.50
generic_CBS651218.51127.2e35.4N.D.325.0N.D.N.D.
CCR2_CBS693043.51032.3e111.5N.D.2376.711.80.32
PRR9_CBS583153.41035.0e62.8N.D.2271.012.70.53
  • Plants were grown and assayed as described in Figure 1 and rhythmic parameters calculated according to the methods of Plautz et al. (1997). N.D., not determined.

  • a Expression is defined as luciferase counts/seedling/25 min.

  • b A plant is defined as rhythmic if fast Fourier transform nonlinear least squares analysis returned an RAE <1.

  • c Phase is calculated in circadian time (= estimated phase/period × 24 h).

  • d P values for expression were calculated by comparing reference with its derivative constructs (i.e., comparing each mutant to the construct listed first within each group) using the Kruskal-Wallis test, with Bonferroni correction for multiple testing.

  • e These plants were assayed using a different CCD camera; thus, luciferase activity levels can't be directly compared with those observed with other constructs.