Table 3.

Kanr:Kans Ratios of F1 Progeny from the Delayed Pollination Test

GroupHours after EmasculationProgenyKanr:KansP (Group 1 as Reference)
112 (stage 13)Kanr, 00
Kans, 1219
224 (stage 14)Kanr, 110.98%<0.01
Kans, 1125
348 (stage 15)Kanr, 80.78%<0.01
Kans, 1072
472 (stage 17)Kanr, 70.78%<0.01
Kans, 898
596 (stage 17)Kanr, 133.01%<0.01
Kans, 432
  • Pistils of swa1/+ plants were emasculated at floral stage 12c (Smyth et al., 1990) and pollinated with pollen from wild-type plants at 12 h (stage 13), 24 h (stage 14), 48 h (stage 15), 72 h (stage 17), or 96 h (stage 17) after emasculation. The results from each time point were from three independent plants. Group 1 was a control, because Arabidopsis anthesis occurs naturally at stage 13. Groups 2 to 5 all exhibited significant differences from group 1, indicating that a small fraction of swa1 mutant ovules could be fertilized and produce seeds when pollination was delayed.