Table 1.

GC-MS Data of Products Obtained in CYP90C1 and CYP90D1 Reconstitution Assays

EnzymeSubstrateProductRetention Time (min)Characteristic Ions m/z (Relative Intensity Percentage)
CYP90C122-OHCR22,23-OHCR10.34529 (0.6), 438 (4.1), 243 (8.1), 129 (100)
synthetic10.37529 (0.3), 438 (3.3), 243 (7.8), 129 (100)
22-OH-4-en-3-one22,23-OH-4-en-3-one11.55454 (2.1), 281 (3.1), 229 (25.9), 124 (100)
synthetic11.53454 (1.0), 281 (1.2), 229 (25.8), 124 (100)
22-OH-3-one6-deoxo3DT10.72456 (3.4), 283 (7.7), 231(100), 155 (52.5)
synthetic10.70456 (1.4), 283 (3.7), 231(100), 155 (52.3)
3-epi-6-deoxoCT6-deoxoTY9.68530 (0.3), 440 (8.0), 425 (18.8), 215 (100)
synthetic9.68530 (0.2), 440 (6.7), 425 (17.2), 215 (100)
6-deoxoCT6-deoxoTE10.46530 (0.8), 516 (4.8), 425 (7.4), 215 (100)
synthetic10.46530 (0.1), 516 (4.5), 425 (8.5), 215 (100)
CYP90D122-OHCR22,23-OHCR10.35529 (0.1), 438 (4.1), 243 (7.3), 129 (100)
synthetic10.37529 (0.3), 438 (3.3), 243 (7.8), 129 (100)
22-OH-4-en-3-one22,23-OH-4-en-3-one11.51454 (2.1), 281 (3.3), 229 (25.9), 124 (100)
synthetic11.53454 (1.0), 281 (1.2), 229 (25.8), 124 (100)
22-OH-3-one6-deoxo3DT10.70456 (1.7), 283 (6.3), 231(100), 155 (54.5)
synthetic10.70456 (1.4), 283 (3.7), 231(100), 155 (52.3)
3-epi-6-deoxoCT6-deoxoTY9.68530 (0.1), 440 (7.3), 425 (18.6), 215 (100)
synthetic9.68530 (0.2), 440 (6.7), 425 (17.2), 215 (100)
6-deoxoCT6-deoxoTE10.46530 (0.8), 516 (4.6), 425 (7.1), 215 (100)
synthetic10.46530 (0.1), 516 (4.5), 425 (8.5), 215 (100)