GC-MS Data of Products Obtained in CYP90C1 and CYP90D1 Reconstitution Assays
| Enzyme | Substrate | Product | Retention Time (min) | Characteristic Ions m/z (Relative Intensity Percentage) |
|---|---|---|---|---|
| CYP90C1 | 22-OHCR | 22,23-OHCR | 10.34 | 529 (0.6), 438 (4.1), 243 (8.1), 129 (100) |
| synthetic | 10.37 | 529 (0.3), 438 (3.3), 243 (7.8), 129 (100) | ||
| 22-OH-4-en-3-one | 22,23-OH-4-en-3-one | 11.55 | 454 (2.1), 281 (3.1), 229 (25.9), 124 (100) | |
| synthetic | 11.53 | 454 (1.0), 281 (1.2), 229 (25.8), 124 (100) | ||
| 22-OH-3-one | 6-deoxo3DT | 10.72 | 456 (3.4), 283 (7.7), 231(100), 155 (52.5) | |
| synthetic | 10.70 | 456 (1.4), 283 (3.7), 231(100), 155 (52.3) | ||
| 3-epi-6-deoxoCT | 6-deoxoTY | 9.68 | 530 (0.3), 440 (8.0), 425 (18.8), 215 (100) | |
| synthetic | 9.68 | 530 (0.2), 440 (6.7), 425 (17.2), 215 (100) | ||
| 6-deoxoCT | 6-deoxoTE | 10.46 | 530 (0.8), 516 (4.8), 425 (7.4), 215 (100) | |
| synthetic | 10.46 | 530 (0.1), 516 (4.5), 425 (8.5), 215 (100) | ||
| CYP90D1 | 22-OHCR | 22,23-OHCR | 10.35 | 529 (0.1), 438 (4.1), 243 (7.3), 129 (100) |
| synthetic | 10.37 | 529 (0.3), 438 (3.3), 243 (7.8), 129 (100) | ||
| 22-OH-4-en-3-one | 22,23-OH-4-en-3-one | 11.51 | 454 (2.1), 281 (3.3), 229 (25.9), 124 (100) | |
| synthetic | 11.53 | 454 (1.0), 281 (1.2), 229 (25.8), 124 (100) | ||
| 22-OH-3-one | 6-deoxo3DT | 10.70 | 456 (1.7), 283 (6.3), 231(100), 155 (54.5) | |
| synthetic | 10.70 | 456 (1.4), 283 (3.7), 231(100), 155 (52.3) | ||
| 3-epi-6-deoxoCT | 6-deoxoTY | 9.68 | 530 (0.1), 440 (7.3), 425 (18.6), 215 (100) | |
| synthetic | 9.68 | 530 (0.2), 440 (6.7), 425 (17.2), 215 (100) | ||
| 6-deoxoCT | 6-deoxoTE | 10.46 | 530 (0.8), 516 (4.6), 425 (7.1), 215 (100) | |
| synthetic | 10.46 | 530 (0.1), 516 (4.5), 425 (8.5), 215 (100) |