Kinetic Parameters of CYP90C1- and CYP90D1-Catalyzed Reactions with 22-HydroxyBRs
| Km | kcat | kcat/Km | ||
|---|---|---|---|---|
| Enzymes | Substrate | (μM) | (min−1) | (min−1 μM−1) |
| CYP90C1 | 22-OHCR | 19.4 ± 1.18 | 0.11 ± 0.01 | 0.0057 ± 0.0003 |
| 22-OH-4-en-3-one | 4.94 ± 0.02 | 1.40 ± 0.05 | 0.29 ± 0.02 | |
| 22-OH-3-one | 8.61 ± 0.34 | 2.75 ± 0.04 | 0.32 ± 0.01 | |
| 3-epi-6-deoxoCT | 6.61 ± 0.38 | 3.01 ± 0.22 | 0.45 ± 0.018 | |
| 6-deoxoCT | 35.9 ± 1.35 | 0.14 ± 0.004 | 0.0038 ± 0.0002 | |
| CYP90D1 | 22-OHCR | 18.1 ± 1.01 | 0.12 ± 0.01 | 0.0068 ± 0.0008 |
| 22-OH-4-en-3-one | 0.77 ± 0.03 | 1.01 ± 0.07 | 1.32 ± 0.09 | |
| 22-OH-3-one | 0.73 ± 0.04 | 1.27 ± 0.05 | 1.73 ± 0.14 | |
| 3-epi-6-deoxoCT | 1.06 ± 0.03 | 1.10 ± 0.04 | 1.04 ± 0.006 | |
| 6-deoxoCT | 16.9 ± 1.12 | 0.29 ± 0.01 | 0.017 ± 0.001 |