Table 2.

Kinetic Parameters of CYP90C1- and CYP90D1-Catalyzed Reactions with 22-HydroxyBRs

Kmkcatkcat/Km
EnzymesSubstrate(μM)(min−1)(min−1 μM−1)
CYP90C122-OHCR19.4 ± 1.180.11 ± 0.010.0057 ± 0.0003
22-OH-4-en-3-one4.94 ± 0.021.40 ± 0.050.29 ± 0.02
22-OH-3-one8.61 ± 0.342.75 ± 0.040.32 ± 0.01
3-epi-6-deoxoCT6.61 ± 0.383.01 ± 0.220.45 ± 0.018
6-deoxoCT35.9 ± 1.350.14 ± 0.0040.0038 ± 0.0002
CYP90D122-OHCR18.1 ± 1.010.12 ± 0.010.0068 ± 0.0008
22-OH-4-en-3-one0.77 ± 0.031.01 ± 0.071.32 ± 0.09
22-OH-3-one0.73 ± 0.041.27 ± 0.051.73 ± 0.14
3-epi-6-deoxoCT1.06 ± 0.031.10 ± 0.041.04 ± 0.006
6-deoxoCT16.9 ± 1.120.29 ± 0.010.017 ± 0.001