Table 3.

Identification of Bands from an SDS Polyacrylamide Gel with Mustard TAC Proteins

BandMM (kD)ProteinPositionaZbΔMcxcorrdAGI Acc. No.e
119–21Thioredoxin, putative79–902−0.24.35At3g06730
80–902−0.54.04
132–1422−0.33.83
148–1612−0.14.16
Ribosomal protein L29p97–10311.11.68At5g65220
222–23Hypothetical protein140–1562−1.22.50At2g05430
324–25Ribosomal protein S392–1012−0.33.19ArthCp061
184–19210.41.71
426–28?
530–32Hypothetical protein140–1562−0.42.51At2g05430
633–35PTAC4 (PspA/IM30 family protein)146–15220.21.54At1g65260
223–2412−0.43.03
246–26721.42.70
268–28420.93.14
PTAC6 (expressed protein)272–2792−1.12.59At1g21600
736–38?
839–40PTAC1 (DNA binding protein p24-related)81–892−0.72.55At1g14410
181–19921.23.78
248–2632−1.52.93
PTAC11 (DNA binding protein p24-related)196–2042−0.42.82At2g02740
942–44PTAC14 (SET)90–9610.81.58At4g20130
PTAC11 (DNA binding protein p24-related)85–932−0.72.55At2g02740
96–10310.41.53
104–11621.22.56
120–1342−1.14.15
196–2042−0.42.63
253–25910.11.91
260–26820.73.11
1054–55Iron superoxide dismutase (FE-SOD1)253–2622−0.42.92At5g51100
pfkB-type carbohydrate kinase family protein (PFKB1)578–5972−0.22.70At1g69200
PTAC12 (unknown protein)127–14220.12.53At2g34640
249–2593−0.73.51
1160–62RNA polymerase β″-chain (RpoC2)1083–10912−0.73.22ArthCp012
PTAC9 (expressed protein)121–1302−0.13.20At4g20010
288–2992−0.13.12
1270–72PTAC6 (expressed protein)149–1612−0.53.02At1g21600
189–2082−0.23.36
209–21310.01.62
273–27910.31.54
303–30710.61.55
pfkB-type carbohydrate kinase family protein (PFKB2)146–1602−0.63.20At3g54090
165–17010.21.57
1378–80RNA polymerase β′-chain (RpoC1)327–3412−0.73.28ArthCp013
461–4742−1.44.02
595–6052−1.03.01
PTAC6 (expressed protein)149–1612−0.72.69At1g21600
Hypothetical protein140–14520.02.60At2g05430
PTAC4 (PspA/IM30 family protein)99–1132−0.22.59At1g65260
246–26720.44.76
268–28421.43.62
Mur ligase family protein570–58020.72.66At1g63680
1482–84PTAC10 (S1 domain-containing protein)593–60620.03.52At3g48500
609–6222−1.02.52
1585–87PTAC5 (peptidoglycan binding domain-containing protein)80–922−1.03.26At4g13670
1688–91PTAC5 (peptidoglycan binding domain-containing protein)80–922−0.23.56At4g13670
1795–100PTAC11 (DNA binding protein p24-related)104–1162−0.93.68At2g02740
120–1342−0.13.48
196–2042−1.32.55
18135–145PTAC10 (S1 domain-containing protein)41–462−0.33.18At3g48500
72–7610.11.53
95–11420.73.47
172–17710.21.61
383–40320.22.84
484–48910.21.62
19150–160PTAC3 (SAP domain-containing protein)496–5152−0.53.92At3g04260
703–7132−0.32.85
618–63120.24.22
RNA polymerase β″-chain (RpoC2)1083–109120.02.63ArthCp012
1144–11512−0.52.91
1206–121421.12.53
20165–175RNA polymerase β-chain (RpoB)646–9772−0.23.29ArthCp014
RNA polymerase β″-chain (RpoC2)138–1502−0.23.78ArthCp012
266–2762−0.22.53
511–5252−0.13.09
1144–11512−0.62.74
  • Analysis of MS/MS spectra is based on the Arabidopsis database (ftp.arabidopsis.org/home/tair/sequences/blast_datasets/). MM, molecular mass in kilodaltons.

  • a Position of identified peptides within the database protein sequence.

  • b The charge state of the measured ion (z).

  • c The calculated deviation of the experimentally determined mass from the theoretical average mass of the peptide.

  • d xcorr value calculated by using the sequest algorithm.

  • e NCBI/AGI accession numbers.