Table 4.

Pigment Analysis and Fluorescence Measurements of Wild-Type and Mutant Lines

Pigment CompositionChlorophyll Fluorescence Measurements
LineChlorophyll a/b (mol/mol)Carotenoids/Chlorophyll (mol/mol)VAZ/Chlorophyll (mmol/mol)Fv/Fm(Fm′ − Ft)/Fm′NPQ
Wild type3.2 ± 0.0340.25 ± 0.00840.7 ± 1.40.854 ± 0.0120.719 ± 0.0370.456 ± 0.030
tac22.8 ± 0.1060.35 ± 0.01299.1 ± 4.80.497 ± 0.0550.365 ± 0.0390.890 ± 0.226
tac64.4 ± 0.2861.48 ± 0.071467.9 ± 18.00.424 ± 0.0890.371 ± 0.0730.324 ± 0.058
tac122.8 ± 0.0570.60 ± 0.042216.7 ± 13.50.546 ± 0.0130.395 ± 0.0220.399 ± 0.044
  • Pigment data are the average of three independent samples. Flourescence quenching analysis is averaged from nine plants. VAZ is the xanthophyll pool size (violaxanthin + antheraxanthin + zeaxanthin).