Table 3.

Sensitivity to NLP Is Restricted to Dicotyledonous Plant Cells

Cell TypeNLP ConcentrationSurvivors Relative to Control (%) (±sd)
COS-7
    (DMEM)1 μM NLPPp57.5 ± 0.5
    (Quantum 333)1 μM NLPPp85.5 ± 6.5
Human fibroblasts (GM5756)
    (DMEM)1 μM NLPPp85.5 ± 7.5
    (Quantum 333)1 μM NLPPp120.5 ± 17.5
Sheep erythrocytes (PBS)1 μM NLPPp102.3 ± 4.7
    − Ca2+2 μM NLPPya97.7 ± 2.9
Sheep erythrocytes (PBS)1 μM NLPPs98.4 ± 5.7
    + Ca2+2 μM NLPPya96.4 ± 12.1
P. pastoris spheroplasts1 μM NLPPs86.2 ± 9.5
2 μM NLPPya52.0 ± 4.3
P. patens2 μM NLPPp100.7 ± 11.8
Maize cell culture0.1 μM NLPPya112.3 ± 2.6
1 μM NLPPya91.1 ± 2.8
Arabidopsis cell culture1 μM NLPPya40.3 ± 4.6
Parsley cell culture1 μM NLPPya7.2 ± 0.8
Maize protoplasts0.1 μM NLPPp88.3 ± 11.8
Arabidopsis protoplasts1 μM NLPPp89.7 ± 8.4
0.1 μM NLPPya102.2 ± 15.7
1 μM NLPPya97.2 ± 7.5
0.1 μM NLPPp5.7 ± 2.4
1 μM NLPPp3.1 ± 1.6
0.1 μM NLPPya4.2 ± 0.9
1 μM NLPPya1.0 ± 0.9
Parsley protoplasts0.1 μM NLPPp4.4 ± 0.8
1 μM NLPPp2.2 ± 1.0
0.1 μM NLPPya2.2 ± 0.9
1 μM NLPPya0.6 ± 0.6
  • Various cell types were treated with different NLP preparations at the concentrations indicated. Cell death rates were determined at times and using protocols described in Methods. Values represent average values ± sd.