Table 1.

GC-MS Profiles of GA Metabolites by Recombinant EUIa

SampleRetention Time on GC in min (KRI)bCharacteristic Ions, m/z (Relative Intensity)c
GA4 metabolited10.85 (2927)581 [M+-15] (2), 493 (100), 299 (3), 217 (14), 181 (7), 147 (30), 129 (13), 73 (95)
Authentic 16α,17-[OH]2 GA4d10.87 (2929)581 [M+-15] (2), 493 (100), 299 (4), 217 (9), 181 (10), 147 (25), 129 (12), 73 (90)
Authentic 16β,17-[OH]2 GA4d10.58 (2895)581 [M+-15] (2), 493 (100), 299 (4), 217 (6), 181 (9), 147 (21), 129 (10), 73 (87)
GA9 metabolited9.77 (2792)493 [M+-15] (2), 405 (100), 345 (2), 301 (4), 217 (10), 183 (14), 147 (29), 73 (76)
Authentic 16α,17-[OH]2 GA9d9.80 (2793)493 [M+-15] (2), 405 (100), 345 (2), 301 (4), 217 (10), 183 (15), 147 (25), 73 (77)
Authentic 16β,17-[OH]2 GA9d9.60 (2769)493 [M+-15] (3), 405 (100), 345 (2), 301 (4), 217 (6), 183 (13), 147 (21), 73 (77)
GA12 metabolited9.80 (2797)523 [M+-15] (5), 506 (7), 448 (6), 435 (60), 416 (10), 375 (22), 239 (20), 147 (28), 73 (100)
Authentic 16α,17-[OH]2 GA12d9.80 (2796)523 [M+-15] (3), 506 (4), 448 (5), 435 (60), 416 (6), 375 (26), 239 (17), 147 (30), 73 (100)
  • a Metabolites were purified in the presence of 1% acetic acid.

  • b Kovats retention index.

  • c m/z, mass-to-charge ratio.

  • d Methyl ester-trimethylsilyl (TMSi) ether derivative.