Table 3.

Levels of 16α,17-Dihydroxy GAs in Eui-OX and Wild-Type Plants

SampleIons Used for Quantification, m/z (Relative Intensity)aRelative Levelb
Wild-typeEndogenous: 365 [M-H] (100), 347 (8), 321 (8)1
16α,17-[OH]2 GA12cInternal standard (2H2): 367 [M-H] (100), 349 (8), 323 (7)
Eui-OXEndogenous: 365 [M-H] (100), 347 (10), 321 (7)18
16α,17-[OH]2 GA12cInternal standard (2H2): 367 [M-H] (100), 349 (8), 323 (7)
Wild-typeEndogenous: 493 [M+-15] (5), 405 (100), 345 (4)1
16α,17-[OH]2 GA9dInternal standard (2H3): 496 [M+-15] (3), 408 (100), 348 (2)
Eui-OXEndogenous: 493 [M+-15] (3), 405 (100), 345 (3)12
16α,17-[OH]2 GA9dInternal standard (2H3): 496 [M+-15] (3), 408 (100), 348 (2)
  • a Only representative ions are shown. The intensity of the most abundant ion was set as 100.

  • b The values for wild-type samples were arbitrarily set as 1.

  • c Determined without derivatization on LC-MS/MS.

  • d Determined as methyl ester-TMSi ether derivatives on GC-selected ion monitoring. The 16α,17-[OH]2 GA4 could not be quantified due to its low abundance in both samples and comigration of impurities.