Table 2.

Interactions among MADS Box, LUG, and SEU Proteins in Yeast

Two-Hybrid Assay-LWH-LWH + 3AT
pBD-AGL24 + pAD-AGL24++ (3 mM)
pBD-AGL24 + pAD-SVP
pBD-AGL24 + pAD-LUG
pBD-AGL24 + pAD-SEU
pBD-SVP + pAD-AGL24
pBD-SVP + pAD-SVP
pBD-SVP + pAD-LUG
pBD-SVP + pAD-SEU
pBD-AP1Δ1 + pAD-AGL24++ (3 mM)
pBD-AP1Δ1+ pAD-SVP++ (1 mM)
pBD-AP1Δ1+ pAD-LUG
pBD-AP1Δ1+ pAD-SEU
pBD-LUG + pAD-AGL24
pBD-LUG + pAD-SVP
pBD-LUG + pAD-LUG
pBD-LUG + pAD-SEU++ (5 mM)
Three-Hybrid Assay-LWAH-LWAH + 3AT
pBD-AGL24 + pTFT-AP1 + pAD-SEU+
pBD-AGL24 + pTFT-empty + pAD-SEU
pBD-AGL24 + pTFT-AP1 + pAD-empty
pBD-SVP + pTFT-AP1 + pAD-SEU+
pBD-SVP + pTFT-empty + pAD-SEU
pBD-SVP + pTFT-AP1 + pAD-empty
Four-Hybrid Assay-LWAUH-LWAUH + 3AT
pBD-SVP + pTFT-AP1
pRED-SEU + pAD-LUG+
pBD-empty + pTFT-AP1
pRED-SEU + pAD-LUG
pBD-SVP + pTFT-empty
pRED-SEU + pAD-LUG
pBD-SVP + pTFT-AP1
pRED-empty + pAD-LUG
pBD-SVP + pTFT-AP1
pRED-SEU + pAD-empty
  • Interactions among SVP, AGL24, AP1, SEU, and LUG detected in yeast. cDNAs cloned in pBD are fused in frame with the DNA BD of GAL4, cDNAs cloned in pAD are fused to the GAL4 AD, and the cDNAs cloned in pTFT and pRED are fused to a nuclear localization signal. The yeast strains were plated on different selective media (without His) and with different concentrations of 3-aminotriazole (3AT). The minus sign indicates no growth and the plus sign indicates growth on selective media.