Table 2.

Phenotypes of Empty Vector Control and CHS-Silenced Roots

PhenotypeEmpty Vector Control (Mean ± se)CHS-Silenced (Mean ± se)P Level
Number of hairy roots per plantlet after 26 d3.22 ± 0.27 (n = 55)3.16 ± 0.33 (n = 49)0.85
Hairy root length at 26 d (mm)22.8 ± 2.07 (n = 166)22.3 ± 2.18 (n = 125)0.43
Hairy root width at 26 d, 1 cm behind root tip (mm)1.08 ± 0.06 (n = 40)1.10 ± 0.06 (n = 40)0.35
Root hair length at 26 d (mm)1.33 ± 0.04 (n = 20)1.42 ± 0.04 (n = 20)0.16
Number of lateral roots per root at 26 d1.06 ± 0.13 (n = 166)0.77 ± 0.14 (n = 125)0.07
Number of nodules per root in Perlite (rhizobia grown with luteolin)0.46 ± 0.06 (n = 85)0.06 ± 0.02 (n = 83)a<0.01
Number of nodules per root on agar (rhizobia grown without luteolin)1.05 ± 0.29 (n = 20)0.1 ± 0.05 (n = 43)a<0.01
Number of nodules per root on agar (rhizobia grown with luteolin)0.63 ± 0.13 (n = 30)0.03 ± 0.01 (n = 31)a<0.01
Number of nodules per complemented root on agar (rhizobia grown with luteolin)0.76 ± 0.15 (n = 39)0.60 ± 0.11 (n = 35)a0.18
  • a All nodules found on CHS-silenced plantlets were located on roots that showed fluorescence in a screen for fluorescence at the end of the nodulation experiment.