Table 1.

Analysis of Elongation, Dehiscence, and ARF8 Expression Ratios in Lines Used in This Study

LineSilique LengthaElongationbDehiscencebmRNA Expression Ratioc
Ler4.5 mm ± 0.5 mm1.0
arf8-47.5 mm ± 0.8 mm+++++1.6 ± 0.2
Col3.7 mm ± 0.5 mm1.0
arf8-4NIL5.9 mm ± 0.7 mm++++1.3 ± 0.1
arf8-54.0 mm ± 0.6 mm+2.4 ± 0.2
arf8-65.2 mm ± 0.2 mm++++0.6 ± 0.2
Ws3.8 mm ± 0.3 mm1.0
arf8-15.0 mm ± 0.1 mm++++0.2 ± 0.1
arf8-4 + ARF8 #15.4 mm ± 0.8 mm+++4.1 ± 1.2
arf8-4 + ARF8 #25.1 mm ± 0.6 mm+++1.0 ± 0.1
arf8-4 + ARF8 #34.3 mm ± 0.7 mm++ND
arf8-4 + ARF8 #45.6 mm ± 0.6 mm++++1.2 ± 0.2
arf8-4 + ARF8 #55.0 mm ± 0.7 mm+++3.0 ± 0.2
arf8-4 + ARF8 #64.9 mm ± 0.6 mm+++ND
arf8-4 + ARF8 #75.4 mm ± 0.6 mm+++5.9 ± 0.3
arf8-4 + ARF8 #85.7 mm ± 0.5 mm++++ND
arf8-4 + ARF8 #96.0 mm ± 0.8 mm++++ND
Ler + ARF8 #13.8 mm ± 0.7 mmND
Ler + ARF8 #24.3 mm ± 0.4 mmND
Ler + ARF8 #34.6 mm ± 0.4 mmND
  • a Pistil lengths measured 7 d after emasculation (±sd; a minimum of 40 flowers was emasculated and measured for each line).

  • b −, no elongation/dehiscence; +, some elongation; +++/++, very good/good elongation/dehiscence.

  • c The expression ratio of ARF8 mRNA was determined from flowers at anthesis. The ratio for each line is determined in relation to the expression in the corresponding wild-type line, which was set to 1.0 as the reference point. ND, not determined.