Table 3.

GA and ABA Metabolic and Response Gene Expression in the Embryo and Endosperm during Germination

EmbryoEndosperm
Affymetrix IDNamePACABACONPACABACON
GA metabolism
255461_atCPS (GA1)58.5 ± 7.215.4 ± 2.5*10.1 ± 1.8*12.0 ± 3.3*9.2 ± 3.9*6.3 ± 2.8*
262891_atKS (GA2)21.5 ± 2.527.6 ± 3.825.0 ± 5.122.8 ± 1.823.1 ± 0.520.2 ± 2.2
246864_atKO1 (GA3)1053.8 ± 83.2487.3 ± 33.1400.8 ± 7.01231.8 ± 140.3572.8 ± 55.3291.3 ± 42.7
264586_atKAO130.2 ± 1.951.2 ± 1.225.8 ± 0.625.8 ± 5.129.5 ± 4.120.7 ± 1.5
254065_atGA20OX1335.1 ± 14.128.1 ± 1.437.4 ± 4.5204.1 ± 16.623.7 ± 2.914.0 ± 6.0
248371_atGA20OX2306.1 ± 4.27.7 ± 1.0*21.5 ± 3.4203.9 ± 12.68.5 ± 0.7*5.8 ± 3.5*
250611_atGA20OX3775.9 ± 22.324.1 ± 3.73.4 ± 1.5*798.3 ± 29.019.2 ± 1.2*10.7 ± 4.8*
261768_atGA3OX1 (GA4)1984.1 ± 103.4282.8 ± 28.1597.7 ± 89.6642.6 ± 103.586.9 ± 32.8102.8 ± 21.9
260300_atGA3OX2339.4 ± 38.3105.2 ± 6.3376.1 ± 15.0107.8 ± 28.037.3 ± 9.475.5 ± 31.1
260023_atGA2OX210.4 ± 4.2*26.4 ± 6.824.2 ± 10.05.7 ± 3.1*22.9 ± 4.118.8 ± 2.9*
GA signaling
262850_atGAI137.0 ± 4.1286.5 ± 19.773.1 ± 2.1158.1 ± 15.3311.0 ± 30.9218.0 ± 18.2
266331_atRGA87.3 ± 2.790.3 ± 5.374.2 ± 6.988.3 ± 11.969.2 ± 11.647.3 ± 8.6
260141_atRGL113.0 ± 2.6*14.1 ± 2.4*24.6 ± 1.843.5 ± 3.519.3 ± 2.3*37.5 ± 5.3
259042_atRGL2403.9 ± 16.3488.1 ± 43.0167.2 ± 7.9561 ± 58.9715.0 ± 13.9519.2 ± 49.0
246432_atRGL384.2 ± 6.6150.3 ± 9.542.9 ± 1.681.4 ± 5.2100.0 ± 11.275.5 ± 5.4
254160_atSLY1137.2 ± 28.986.4 ± 10.780.8 ± 6.2187.2 ± 2.873.5 ± 10.1102.8 ± 25.3
251200_atATGID1a4.3 ± 1.5*2.7 ± 1.6*6.5 ± 0.6*7.6 ± 2.6*4.5 ± 2.8*11.1 ± 6.3*
259302_atATGID2124.1 ± 11.180.3 ± 6.137.4 ± 2.6102.7 ± 8.785.4 ± 11.653.0 ± 10.8
246782_atATGID324.2 ± 3.327.3 ± 4.310.9 ± 1.4*22.2 ± 4.3*15.6 ± 5.7*16.6 ± 2.2*
ABA metabolism
247025_atABA1397.8 ± 5.1365.2 ± 14.8138.2 ± 2.0260.4 ± 10.6320.0 ± 54.4213.8 ± 14.3
257280_atNCED34.4 ± 2.9*2.1 ± 1.3*2.0 ± 1.5*2.3 ± 1.4*1.5 ± 0.4*1.2 ± 0.3*
257242_atNCED60.9 ± 0.2*1.3 ± 0.3*0.9 ± 1.2*2.1 ± 0.5*1.6 ± 0.5*1.9 ± 0.4*
260797_atNCED97.7 ± 0.7*2.8 ± 1.0*3.3 ± 1.7*21.8 ± 2.026.7 ± 2.524.7 ± 3.7
259669_atABA282.0 ± 5.476.3 ± 4.378.9 ± 1.7116.6 ± 6.6136.0 ± 5.0121.3 ± 2.7
246325_atABA352.5 ± 4.951.5 ± 7.115.7 ± 3.0*36.3 ± 1.236.5 ± 1.933.1 ± 5.6
263570_atAAO374.4 ± 4.173.3 ± 6.842.2 ± 5.796.7 ± 28.694.2 ± 4.0103.0 ± 3.8
266778_atCYP707A253.0 ± 1.582.9 ± 12.325.6 ± 2.919.3 ± 3.5*176.9 ± 17.521.2 ± 10.9
ABA signalingb
253994_atABI1373.9 ± 24.5390.5 ± 53.5436.9 ± 20.3282.6 ± 21.6429.5 ± 143.1640.6 ± 112.3
247957_atABI211.2 ± 5.1*42.6 ± 1.717.1 ± 3.6*3.2 ± 1.7*39.0 ± 4.27.1 ± 2.8*
256898_atABI3264.8 ± 22.4137.3 ± 11.430.6 ± 2.9342.7 ± 29.9190.2 ± 14.1131.7 ± 31.6
263377_atABI469.5 ± 8.8115.3 ± 22.261.6 ± 14.713.9 ± 2.6*42.3 ± 12.9c24.5 ± 6.2*
263907_atABI5182.4 ± 4.8133.7 ± 18.323.7 ± 1.8*108.2 ± 8.8121.2 ± 66.152.2 ± 20.3
258666_atABI8132.6 ± 4.6161.9 ± 12.9122.1 ± 11.6104.9 ± 13.4109.1 ± 16.2112.7 ± 7.0
248719_atrbohD78.3 ± 9.279.8 ± 7.127.5 ± 1.4649.3 ± 7.8501.3 ± 78.9869.9 ± 56.1
262344_atrbohF7.9 ± 3.0*6.2 ± 1.1*17.0 ± 4.3*3.5 ± 1.1*6.1 ± 2.3*21.4 ± 3.6*
Light signaling
264508_atPHYA1385.7 ± 52.3842.1 ± 50.21036.0 ± 53.52516.9 ± 190.11475.2 ± 164.22545.5 ± 145.9
266065_atPHYB331.5 ± 25.5261.2 ± 5.7188.9 ± 1.6299.0 ± 6.0206.6 ± 6.1198.1 ± 8.4
245487_atPHYD137.8 ± 1.9159.9 ± 19.846.0 ± 1.958.8 ± 4.667.9 ± 1.825.7 ± 5.8
254680_atPHYE161.6 ± 8.2180.2 ± 3.4100.5 ± 2.9143.1 ± 19.3176.0 ± 4.282.1 ± 14.9
265584_atPIL5/PIF1373.7 ± 22.6337.2 ± 6.0207.2 ± 11.0383.5 ± 41.5320.4 ± 10.6213.8 ± 29.6
246212_atSPATULA54.5 ± 4.354.7 ± 3.5207.1 ± 4.4187.2 ± 21.4170.7 ± 48.1483.6 ± 121.5
  • Asterisk indicates an absent call in at least two of three replicates. CON, control.

  • a The Arabidopsis homologues of the recently identified rice GA receptor GIBBERELLIN INDEPENDENT DWARF1.

  • b Major and relevant ABA response genes shown only.

  • c One absent, one present, and one marginal call.